AfCS Yeast 2-Hybrid: Interaction Data
Protein short name:
Protein name/synonyms:
Entrez Gene ID:
AfCS ID:
Intact protein length:
1 This column shows the bait
name and the amino (N)- and carboxy (C)-terminal amino acid
coordinates of the bait protein region used to isolate the interacting
prey protein. Fragments of proteins representing folded domains are
often more effective than the full-length protein in identifying
physiologically relevant interactions in this assay. If the domain
structure of a given bait protein was already established, specific
baits were designed to represent one or more folded domains. In cases
where domain structure was not available, a variety of secondary
structure prediction algorithms were used to predict domains and thus
direct bait design.
2Top AfCS BLAST hit obtained by
comparing the bait sequence against the AfCS database, restricted to
hits with at least 90% identity over 90% of the sequence.
3 This column shows the prey
name and the amino (N)- and carboxy (C)-terminal amino acid
coordinates of the prey protein region identified in the screen. In
cases where the nucleotide sequence of the prey fragment began
upstream of the initiation codon, the N-terminal coordinate is 1.
Similarly, in cases where the nucleotide sequence of the prey fragment
finishes downstream of the termination codon, the C-terminal
coordinate corresponds to the last amino acid of the protein.
4Top AfCS BLAST hit obtained by
comparing the prey sequence against the AfCS database, restricted to
hits with at least 90% identity over 90% of the sequence.
5
This column denotes the activation domain library used for identifying
the bait-prey interaction. Screening of an activation domain library
derived from a specific cell type may permit the identification of
protein interactions that are unique to that cell type. The AfCS has
isolated mRNA from several sources, which Myriad has used to
prepare the following specific activation domain libraries:
- Macrophage (Resting RAW264.7 cells + RAW264.7 cells stimulated with a
cocktail of lipopolysaccharide (5mg/ml) and interferon gamma (5nM) for
18 hours + Murine primary bone marrow-derived macrophages)
- Embryo (derived from mRNA isolated from mouse embryos at multiple
developmental stages)
- B cell (Resting primary B cells + stimulated primary B cells (4 hour
incubation with anti-IgM [0.06 microM], anti-CD40 [0.06 microM], and
IL-4 [0.011 nM] + WEHI-231 B cell line)
- Primary cardiac myocytes (Note that this library contains high levels of
mitochondrial DNA and consequently gives fewer interactions)
- Nk-TAg myocyte cell line
6
Date on which interaction was first identified
| 
