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AfCS Yeast 2-Hybrid Interaction Data Overview

2/28/05 A further 59 new Y2H interactions were released 2/28/05. They involve 17 of the baits selected by the AfCS (GRK2, CALM, CAML, FCER1G, ITK, ITPR1, PIK3CA, PIK3CG, PIP5K2C, PLCD1, PLCE1, PIK3R5, PPP2R1A, PTEN, RGS19, SOS and NTAL). 56 prey proteins were pulled out by these baits. Interactions of note were both Grk2 and PI3Kp110a with the p105 subunit of NFkB, calmodulin with several myosin isoforms and NOS2, PP2A with protein kinase N and 2 striatin protein family members and NTAL with a calcium modulating ligand. 53 proteins appeared in the AfCS data set for the first time in this release.

1/31/05 44 new Y2H interactions were released on 1/31/05. They involve 14 of the baits selected by the AFCS (BLNK, GAB2, GRB2, ITPKC, LYNa, PIK3CA, PIK3CG, PIK3R1(724), PIK3R2, PLCD1,PRKCZ, SYK, TRAF1 and VAV1). 26 individual prey proteins were pulled out by these baits. Among these, signaling proteins of note were 14-3-3 zeta, Grb2, Chk2, Btk, Gab2, SHIP, PI3Kp85a and beta-catenin. 8 proteins appear in the AfCS Y2H data set for the first time in this release.

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