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Lipidomics data
The following links connect the user to AfCS lipid arrays. These
arrays present a qualitative map of lipid species fluctuation after
ligand stimulation as the experiment evolves through time. The second
column in each array contains the m/z (mass-to-charge) ratio of peaks
observed in the mass spectrum, while the first displays lipid species
identified with CID MS/MS. The remaining columns display the number
of experiments in which the peak was observed as increasing (positive
number) or decreasing (negative number) out of the total number of
experiments preformed (listed in the Lipid Array heading.)
Statistically significant species/time point combinations are color
coded with red indicating increasing species and blue indicating
decreasing species.
Each experimental condition produces two arrays, representing the
positive and negative instrument modes. Various lipid molecules tend
to form ions at different efficiencies within the two instrument
modes, with phosphatidylcholine (PC), and sphingomyelin (SM) primarily
forming positive ions and phosphatidic acid (PA), phosphatidylinositol
(PI), phosphatidylglycerol (PG), diacylglycerol (DAG), and ceramides
(cer) forming primarily negative ions. Phosphatidylethanolamine (PE)
and phosphatidylserine (PS) ionize well in both modes. The numbers
xx:y preceding the lipid name indicate the total number of carbons and
double bonds in the fatty acid residues at the sn1 and sn2 positions
within the molecule. Species of PC and PE modified with an e or p
designator indicate molecules where one of the fatty acids is present
in an ether or vinyl ether linkage respectively. Additional
information on the AfCS Lipid Arrays is available here.
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