plasmid - information

AfCS Vectors for Multi-Gene RNAi

AfCS investigators published a methodology article in BMC Molecular Biology on 30th October 2007 describing a vector platform for multi-gene RNA interference (BMC Molecular Biology 2007, 8:98). The plasmids described in this study have been distributed to the ATCC, and are available either individually or all together in plate format (ATCC ID for the plate: MBA-330). More specific details on these plasmids are included in the excel file available here. Plasmid barcodes from the excel file can be used in a keyword search to obtain additional plasmid details including maps. Contact ATCC molecular biology technical support for further information or to obtain the plasmids (tech@atcc.org).

AfCS Vectors for Inducible RNAi

AfCS investigators published a report in the 12th September 2006 issue of PNAS describing a lentiviral vector platform for inducible RNA interference. The plasmids described in this study have been distributed to the ATCC, and are available either individually or all together in plate format (ATCC ID for the plate: MBA-268). More specific details on these plasmids are included in the excel file available here. Plasmid barcodes from the excel file can be used in a keyword search to obtain additional plasmid details including maps. Contact ATCC molecular biology technical support for further information or to obtain the plasmids (tech@atcc.org).

More than 3,000 AfCS mouse clones are now available from the ATCC

SEARCH FOR INFORMATION ABOUT THE CLONES WITH BLAST OR KEYWORD SEARCHES

Use the Blast option when you have a nucleotide sequence.

Use the keyword search when you have textual information about the construct (e.g., gene name). If you already have an ATCC ID or AfCS barcode you should use the keyword search option.

BROWSE THE LIST OF PLASMID CONSTRUCTS

Browse those plasmids that can be ordered through the ATCC. At this time, only some of the clones on the list have been assigned ATCC id numbers and only some of the corresponding links to the ATCC are operable. The ATCC has assured us that they will process clones that do not yet have ATCC ids assigned, but additional processing time will be required. Clones lacking ATCC ids should be referenced by their AfCS barcode.

ORDERING INFORMATION

After you have obtained an ATCC ID or AfCS barcode, an order can be placed through the ATCC. To obtain information on ordering from the ATCC follow the link to Ordering Info.