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Blast Record Section

Blast Record Categories

E-Value

This is a statistical measure of the sequence similarity as reported by the Blast algorithm. In general, a lower E-value (i.e. one that is closer to zero) means a stronger similarity.

Score

This is a score of the sequence similarity as reported by the Blast algorithm. In general, a higher score means a stronger similarity.

Identities

The number of identical sequences and their percentage in the aligned region of the Blast match.

Positives

The number of positive sequence matches and their percentage in the aligned region of the Blast match.

Query Match Length

The length and percentage of the query (Molecule Page) sequence in the Blast match.

Subject Match Length

The length and percentage of the subject (hit) sequence in the Blast match.

Gaps

The number of gaps in the aligned region of the Blast match.

Representative Title

A representative title for this sequence, which may not be entirely reflective of all the protein records which contain this sequence.

Alignment

Displays a pairwise alignment between the Molecule Page sequence and the Blast record that is matched.

Database

The name of the database that is the source of the protein record. Most of these are self-explanatory. The UniProt (SwissProt and TrEMBL) variants are noted variant sequences (either splice variants, variants, or conflicts) of the primary sequence in that record.

GI

The GI number of the protein database record at NCBI. SwissProt records have GI numbers (as they are part of the NCBI Entrez system), but TrEMBL records do not, nor do the UniProt variants. The GI numbers are hyperlinked to NCBI's Entrez browser.

Accession

This is an identifier of the database record. When database records have multiple accessions, this field is the primary accession (i.e. the first one in the accession list). For UniProt sequences, the accession is hyperlinked to PIR's UniProt database browser. For Ensembl records, the accession is hyperlinked to the Protein Report page.

Entry

For UniProt records, the entry field is the unique identifier for the the record, and is hyperlinked to EBI's UniProt database brower. For Ensembl records, the Entry field contains the Ensembl Gene ID related to the protein record, and is hyperlinked to the Ensembl Gene Report page.

Name

For the sequences with a GI number, this is the title used in the Fasta header in their non-redundant protein Fasta file. For UniProt sequences, it is a combination of the description (DE) and organism (OS) lines.

Length

The sequence length in number of amino acids.

Update

The last date the sequence database record was updated by the author in the given database (Genbank, SwissProt, etc.)

Species

The unique species name as given in the NCBI Taxonomy database. This field is hyperlinked to the NCBI Taxonomy browser, where one can see taxonomic hierarchy.