Research library: Techniques
2003: December
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December
technical report
Local gene knockdown in the brain using viral-mediated RNA interference
Jonathan D Hommel, Robert M Sears, Dan Georgescu, Diana L Simmons and Ralph J DiLeone
Nature Medicine 9, 1539–1544
(December 2003); doi:10.1038/nm964
| Abstract | Full Text (HTML/PDF) |
review article
Intravital imaging of cell movement in tumours
John Condeelis and Jeffrey E. Segall
Nature Reviews Cancer 3, 921–930
(December 2003); doi:10.1038/nrc1231
| Abstract | Full Text (HTML / PDF) |
letter
Genetically targeted chromophore-assisted light inactivation
Oded Tour, Rene M Meijer, David A Zacharias, Stephen R Adams & Roger Y Tsien
Nature Biotechnology 21, 1505–1508
(December 2003); doi:10.1038/nbt914
| Abstract
| Full Text (HTML/PDF) |
letters to nature
Optimization of specificity in a cellular protein interaction network by negative selection
Ali Zarrinpar, Sang-Hyun Park and Wendell A. Lim
Nature 426, 676–680
(11 December 2003); doi:10.1038/nature02178
| First Paragraph | Full Text (HTML / PDF) |
news and views
Signal transduction: Molecular monogamy
Drew Endy and Michael B. Yaffe
Nature 426, 614–615
(11 December 2003); doi:10.1038/426614a
| Full Text (HTML / PDF) |
article
Fluorobodies combine GFP fluorescence with the binding characteristics of antibodies
Ahmet Zeytun, Andreas Jeromin, Bethe A Scalettar, Geoffrey S Waldo & Andrew RM Bradbury
Nature Biotechnology 21,1473–1479
(December 2003); doi:10.1038/nbt911
| Abstract | Full Text (HTML/PDF) |
news and views
Fluorescent proteins with ties that bind
Timothy A Ryan
Nature Biotechnology 21,1447–1448
(December 2003); doi:10.1038/nbt1203-1447
| Full Text (HTML/PDF) |
letters
Analyzing antibody specificity with whole proteome microarrays
Gregory A Michaud, Michael Salcius, Fang Zhou, Rhonda Bangham, Jaclyn Bonin, Hong Guo, Michael Snyder, Paul F Predki & Barry I Schweitzer
Nature Biotechnology 4, 1509–1512
(December 2003); doi:10.1038/nbt910
| Abstract | Full Text (HTML/PDF) |
news and views
Cloning by numbers
Louis-Marie Houdebine
Nature Biotechnology 21, 1451–1452
(December 2003); doi:10.1038/nbt1203-1451
| Full Text (HTML/PDF) |
review article
Tissue microarrays in drug discovery
Guido Sauter, Ronald Simon and Kenneth Hillan
Nature Reviews Drug Discovery 2, 962–972
(December 2003); doi:10.1038/nrd1254
| Abstract | Full Text (HTML / PDF) |
November
perspectives
Timeline: Dorothy Hodgkin and her contributions to biochemistry
Judith A. K. Howard
Nature Reviews Molecular Cell Biology 4, 891–896
(November 2003); doi:10.1038/nrm1243
| Abstract | Full Text (HTML / PDF) |
technical report
DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray
Siew Loon Ooi, Daniel D Shoemaker and Jef D Boeke
Nature Genetics 35, 277–286
(November 2003); doi:10.1038/ng1258
| Abstract | Full Text (HTML/PDF) |
news and views
Lethal combinations
Chandra L Tucker and Stanley Fields
Nature Genetics 35, 204–205
(November 2003); doi:10.1038/ng1103-204
| Full Text (HTML/PDF) |
letter
Computational discovery of gene modules and regulatory networks
Ziv Bar-Joseph, Georg K Gerber, Tong Ihn Lee, Nicola J Rinaldi, Jane Y Yoo, François Robert, D Benjamin Gordon, Ernest Fraenke, Tommi S Jaakkola, Richard A Young, & David K Gifford
Nature Biotechnology 21,1337–1342
(November 2003); doi:10.1038/nbt890
| Abstract | Full Text (HTML/PDF) |
letter
Reconstructing genetic networks in yeast
Zhaolei Zhang & Mark Gerstein
Nature Biotechnology 21,1295–1297
(November 2003); doi:10.1038/nbt1103-1295
| Full Text (HTML/PDF) |
article
Targeting cytokines to inflammation sites
Gill Adams, Sandrine Vessillier, Hanna Dreja and Yuti Chernajovsky
Nature Biotechnology 21, 1314–1320
(November 2003); doi:10.1038/nbt888
| Abstract | Full Text (HTML/PDF) |
news and views
Engineering better cytokines
Lawrence Steinman
Nature Biotechnology 21, 1293–1294
(November 2003); doi:10.1038/nbt1103-1293
| Full Text (HTML/PDF) |
article
A genetic approach to inactivating chemokine receptors using a modified viral protein
V McNeil Coffield, Qi Jiang & Lishan Su
Nature Biotechnology 21,1321–1327
( 2000); doi:10.1038/nbt889
| Full Text (HTML/PDF) |
news and views
Cell biology: Enlightened messages
Clare Thomas
Nature 426, 27
(06 November 2003); doi:10.1038/426027a
| Full Text (HTML / PDF) |
October
article
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
Shiaoching Gong, Chen Zheng, Martin L. Doughty, Kasia Losos, Nicholas Didkovsky, Uta B. Schambra, Norma J. Nowak, Alexandra Joyner, Gabrielle Leblanc, Mary E. Hatten and Nathaniel Heintz
Nature 425, 917–925
(30 October 2003); doi:10.1038/nature02033
| Abstract | Full Text (HTML / PDF) |
news and views
Molecular neuroscience: BAC-to-BAC images of the brain
Huda Y. Zoghbi
Nature 425, 907–908
(30 October 2003); doi:10.1038/425907a
| Full Text (HTML / PDF) |
perspectives
Innovation: New tools for functional mammalian cancer genetics
Thijn R. Brummelkamp and René Bernards
Nature Reviews Cancer 3, 781–789
(October 2003); doi:10.1038/nrc1191
| Abstract | Full Text (HTML / PDF) |
review
Soft landing for protein chips
Michael P Washburn
Nature Biotechnology 21, 1156–1157
( 2000); doi:10.1038/nbt1003-1156
| Abstract | Full Text (HTML/PDF) |
review article
Cell-death alternative model organisms: why and which?
Pierre Golstein, Laurence Aubry and Jean-Pierre Levraud
Nature Reviews Molecular Cell Biology 4, 798–807
(October 2003); doi:10.1038/nrm1224
| Abstract | Full Text (HTML / PDF) |
article
Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors
Kyung-Soon Park, Dong-ki Lee, Horim Lee, Yangsoon Lee, Young-Soon Jang, Yong Ha Kim, Hyo-Young Yang, Sung-I Lee, Wongi Seol1 & Jin-Soo Kim
Nature Biotechnology 21, 1208–1214
(October 2003); doi:10.1038/nbt868
| Full Text (HTML/PDF) |
perspectives
Innovation: Target discovery
Mark A. Lindsay
Nature Reviews Drug Discovery 2, 831–838
(October 2003); doi:10.1038/nrd1202
| Abstract | Full Text (HTML / PDF) |
September
research article
Phosphospecific proteolysis for mapping sites of protein phosphorylation
Zachary A. Knight, Birgit Schilling, Richard H. Row, Denise M. Kenski, Bradford W. Gibson and Kevan M. Shokat
Nature Biotechnology 21, 1047–1054
(September 2003); doi:10.1038/nbt863
| Abstract | Full Text (HTML/PDF) |
letter
Activation of the interferon system by short-interfering RNAs
Carol A. Sledz, Michelle Holko, Michael J. de Veer, Robert H. Silverman and Bryan R.G. Williams
Nature Cell Biology 5, 834–839
(September 2003); doi:10.1038/ncb1038
| First Paragraph | Full Text (HTML/PDF) |
news and views
Small-interfering RNAs in the radar of the interferon system
Eric G. Moss and John M. Taylor
Nature Cell Biology 5, 771–772
(September 2003); doi:10.1038/ncb0903-771
| Full Text (HTML/PDF) |
research article
Engineering of a macromolecular scaffold to develop specific protease inhibitors
A. Allart Stoop and Charles S. Craik
Nature Biotechnology 21, 1063–1068
(September 2003); doi:10.1038/nbt860
| Abstract | Full Text (HTML/PDF) |
news and views
Two hands (or four) are better than one
Ben M. Dunn and Jörg Bungert
Nature Biotechnology 21, 1019–1021
(September 2003); doi:10.1038/nbt0903-1019
| Full Text (HTML/PDF) |
review article
The role of α-synuclein in Parkinson's disease: insights from animal models
Eleonora Maries, Biplob Dass, Timothy J. Collier, Jeffrey H. Kordower and Kathy Steece-Collier
Nature Reviews Neuroscience 4, 727–738
(September 2003); doi:10.1038/nrn1199
| Abstract | Full Text (HTML / PDF) |
research article
Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors
Oliver J. Müller, Felix Kaul, Matthew D. Weitzman, Renata Pasqualini, Wadih Arap, Jürgen A. Kleinschmidt and Martin Trepel
Nature Biotechnology 21, 1040–1046
(September 2003); doi:10.1038/nbt856
| Abstract | Full Text (HTML/PDF) |
news and views
AAV display—homing in on the target
André Lieber
Nature Biotechnology 21, 1011–1013
(September 2003); doi:10.1038/nbt0903-1011
| Full Text (HTML/PDF) |
August
research article
Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics
Jeroen Krijgsveld, René F Ketting, Tokameh Mahmoudi, Janik Johansen, Marta Artal-Sanz, C. Peter Verrijzer, Ronald H. A. Plasterk and Albert J. R. Heck
Nature Biotechnology 21, 927–931
(August 2003); doi:10.1038/nbt848
| First Paragraph | Full Text (HTML/PDF) |
research article
Targeting of proteins to membranes through hedgehog auto-processing
Sylvie Vincent, Abraham Thomas, Bradley Brasher and John D Benson
Nature Biotechnology 21, 936–940
(August 2003); doi:10.1038/nbt844
| First Paragraph | Full Text (HTML/PDF) |
research article
A transgenic mouse model of the ubiquitin/proteasome system
Kristina Lindsten, Victoria Menéndez-Benito, Maria G Masucci and Nico P Dantuma
Nature Biotechnology 21, 897–902
(August 2003); doi:10.1038/nbt851
| Abstract | Full Text (HTML/PDF) |
July
research article
Measurement of the millisecond activation switch of G protein-coupled receptors in living cells
Jean-Pierre Vilardaga, Moritz Bünemann, Cornelius Krasel, Marián Castro and Martin J. Lohse
Nature Biotechnology 21, 807–812
(July 2003); doi:10.1038/nbt838
| Abstract | Full Text (HTML/PDF) |
perspectives
Innovation: Looking forward to seeing calcium
Rüdiger Rudolf, Marco Mongillo, Rosario Rizzuto and Tullio Pozzan
Nature Reviews Molecular Cell Biology 4, 579–586
(July 2003); doi:10.1038/nrm1153
| Summary | Full Text (HTML / PDF) |
research article
Phosphoinositide profiling in complex lipid mixtures using electrospray ionization mass spectrometry
Markus R Wenk, Louise Lucast, Gilbert Di Paolo, Anthony J Romanelli, Sharon F Suchy, Robert L Nussbaum, Gary W Cline, Gerald I Shulman, Walter McMurray and Pietro De Camilli
Nature Biotechnology 21, 813–817
(July 2003); doi:10.1038/nbt837
| First Paragraph | Full Text (HTML/PDF) |
article
PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes
Vamsi K Mootha, Cecilia M Lindgren, Karl-Fredrik Eriksson, Aravind Subramanian, Smita Sihag, Joseph Lehar, Pere Puigserver, Emma Carlsson, Martin Ridderstråle, Esa Laurila, Nicholas Houstis, Mark J Daly, Nick Patterson, Jill P Mesirov, Todd R Golub, Pablo Tamayo, Bruce Spiegelman, Eric S Lander, Joel N Hirschhorn, David Altshuler and Leif C Groop
Nature Genetics 34, 267–273
(July 2003); doi:10.1038/ng1180
| Abstract | Full Text (HTML/PDF) |
news and views
PGC-1α at the crossroads of type 2 diabetes
Alan D Attie and Christina M Kendziorski
Nature Genetics 34, 244–245
(July 2003); doi:10.1038/ng0703-244
| Full Text (HTML/PDF) |
June
research article
Discovering potent and selective reversible inhibitors of enzymes in complex proteomes
Donmienne Leung, Christophe Hardouin, Dale L Boger and Benjamin F Cravatt
Nature Biotechnology 21, 687–691
(June 2003); doi:10.1038/nbt826
| First Paragraph | Full Text (HTML/PDF) |
article
Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo
Masato Kanemaki, Alberto Sanchez-Diaz, Agnieszka Gambus and Karim Labib
Nature 423, 720–725
(12 June 2003); doi:10.1038/nature01692
| Abstract | Full Text (HTML / PDF) |
letter
Gene expression phenotypic models that predict the activity of oncogenic pathways
Erich Huang, Seiichi Ishida, Jennifer Pittman, Holly Dressman, Andrea Bild, Mark Kloos, Mark D'Amico, Richard G Pestell, Mike West and Joseph R Nevins
Nature Genetics 34, 226–230
(June 2003); doi:10.1038/ng1167
| First Paragraph | Full Text (HTML/PDF) |
research article
Global protein function prediction from protein-protein interaction networks
Alexei Vazquez, Alessandro Flammini, Amos Maritan and Alessandro Vespignani
Nature Biotechnology 21, 697–700
(June 2003); doi:10.1038/nbt825
| First Paragraph | Full Text (HTML/PDF) |
article
Module networks: identifying regulatory modules and their condition-specific regulators from gene expression data
Eran Segal, Michael Shapira, Aviv Regev, Dana Pe'er, David Botstein, Daphne Koller and Nir Friedman
Nature Genetics 34, 166–176
(June 2003); doi:10.1038/ng1165
| Abstract | Full Text (HTML/PDF) |
review article
Predicting therapeutic value in the lead optimization phase of drug discovery
Terry Kenakin
Nature Reviews Drug Discovery 2, 429–438
(June 2003); doi:10.1038/nrd1110
| Summary | Full Text (HTML / PDF) |
May
letters to nature
Computational design of receptor and sensor proteins with novel functions
Loren L. Looger, Mary A. Dwyer, James J. Smith and Homme W. Hellinga
Nature 423, 185–190
(08 May 2003); doi:10.1038/nature01556
| First Paragraph | Full Text (HTML / PDF) |
news and views
Computational biology: Biosensor design
William F. DeGrado
Nature 423, 132–133
(08 May 2003); doi:10.1038/423132a
| Full Text (HTML / PDF) |
April
review article
Genetics and the making of Homo sapiens
Sean B. Carroll
Nature 422, 849–857
(24 April 2003); doi:10.1038/nature01495
| Summary | Full Text (HTML / PDF) |
feature
A vision for the future of genomics research
Francis S. Collins, Eric D. Green, Alan E. Guttmacher and Mark S. Guyer
Nature 422, 835–847
(24 April ); doi:10.1038/nature01626
| Full Text (HTML / PDF) |
research article
Enhancement of therapeutic protein in vivo activities through glycoengineering
Steve Elliott, Tony Lorenzini, Sheilah Asher, Ken Aoki, David Brankow, Lynette Buck, Leigh Busse, David Chang, Janis Fuller, James Grant, Natasha Hernday, Martha Hokum, Sylvia Hu, Andrew Knudten, Nancy Levin, Renee Komorowski, Frank Martin, Rachell Navarro, Timothy Osslund, Gary Rogers, Norma Rogers, Geri Trail and Joan Egrie
Nature Biotechnology 21, 414–421
(April 2003); doi:10.1038/nbt799
| Abstract | Full Text (HTML/PDF) |
March
perspective
Integration from proteins to organs: the Physiome Project
Peter J. Hunter and Thomas K. Borg
Nature Reviews Molecular Cell Biology 4, 237–243
(March 2003); doi:10.1038/nrm1054
| Summary | Full Text (HTML / PDF) |
review article
Mice, microbes and models of infection
Jan Buer and Rudi Balling
Nature Reviews Genetics 4, 195–205
(March 2003); doi:10.1038/nrg1019
| Summary | Full Text (HTML / PDF) |
research article
A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling
Blagoy Blagoev, Irina Kratchmarova, Shao-En Ong, Mogens Nielsen, Leonard J. Foster and Matthias Mann
Nature Biotechnology 21, 315–318
(March 2003); doi:10.1038/nbt790
| Abstract | Full Text (HTML/PDF) |
February
original paper
Nrf2 is an inhibitor of the Fas pathway as identified by Achilles' Heel Method, a new function-based approach to gene identification in human cells
Kumar U. Kotlo, Furma Yehiely, Elena Efimova, Heather Harasty, Bahar Hesabi, Ksenya Shchors, Paz Einat, Ada Rozen, Eva Berent and Louis P Deiss
Oncogene 22, 797–806
(13 February 2003); doi:10.1038/sj.onc.1206077
| Abstract |
January
article
Evolutionarily conserved networks of residues mediate allosteric communication in proteins
Gürol M. Süel, Steve W. Lockless, Mark A. Wall and Rama Ranganathan
Nature Structural Biology 10, 59–69
(January 2003); doi:10.1038/nsb881
| Abstract | Full Text (HTML/PDF) |
article
Automated design of specificity in molecular recognition
James J. Havranek and Pehr B. Harbury
Nature Structural Biology 10, 45–52
(January 2003); doi:10.1038/nsb877
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