Research library: Techniques
2004: December
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December
letters to nature
Accurate multiplex gene synthesis from programmable DNA microchips
Jingdong Tian, Hui Gong, Nijing Sheng, Xiaochuan Zhou, Erdogan Gulari, Xiaolian Gao and George Church
Nature 432, 1050–1054
(23 December 2004); doi:10.1038/nature03151
| First Paragraph | Full Text (HTML / PDF) |
article
Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity
Stéphanie Hugues, Luc Fetler, Laura Bonifaz, Julie Helft, François Amblard and Sebastian Amigorena
Nature Immunology 5, 1235–1242
(December 2004); doi:10.1038/ni1134
| Abstract | Full Text (HTML/PDF) |
article
Visualizing dendritic cell networks in vivo
Randall L Lindquist, Guy Shakhar, Diana Dudziak, Hedda Wardemann, Thomas Eisenreich, Michael L Dustin and Michel C Nussenzweig
Nature Immunology 5, 1243–1250
(December 2004); doi:10.1038/ni1139
| Abstract | Full Text (HTML/PDF) |
news and views
Lead and follow: the dance of the dendritic cell and T cell
Antonio Lanzavecchia and Federica Sallusto
Nature Immunology 5, 1201–1202
(December 2004); doi:10.1038/ni1204-1201
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research article
Baculovirus expression system for heterologous multiprotein complexes
Imre Berger, Daniel J Fitzgerald and Timothy J Richmond
Nature Biotechnology 22, 1583–1587
(December 2004); doi:10.1038/nbt1036
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news and views
Baculovirus solves a complex problem
Polly Roy
Nature Biotechnology 22, 1527–1528
(December 2004); doi:10.1038/nbt1204-1527
| Full Text (HTML/PDF) |
research article
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Nathan C Shaner, Robert E Campbell, Paul A Steinbach, Ben N G Giepmans, Amy E Palmer and Roger Y Tsien
Nature Biotechnology 22, 1567–1572
(December 2004); doi:10.1038/nbt1037
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news and views
A new harvest of fluorescent proteins
George H Patterson
Nature Biotechnology 22, 1524–1525
(December 2004); doi:10.1038/nbt1204-1524
| Full Text (HTML/PDF) |
article
Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells
Emilia Galperin, Vladislav V Verkhusha and Alexander Sorkin
Nature Methods 1, 209–217
(December 2004); doi:10.1038/nmeth720
| Abstract | Full Text (HTML/PDF) |
technical report
Protein microarrays for multiplex analysis of signal transduction pathways
Steven M Chan, Joerg Ermann, Leon Su, C Garrison Fathman and Paul J Utz
Nature Medicine 10, 1390–1396
(December 2004); doi:10.1038/nm1139
| Abstract | Full Text (HTML/PDF) |
news and views
A new harvest of fluorescent proteins
George H Patterson
Nature Biotechnology 22, 1524–1525
(December 2004); doi:10.1038/nbt1204-1524
| Full Text (HTML/PDF) |
article
Real-time monitoring of ubiquitination in living cells by BRET
Julie Perroy, Stephanie Pontier, Pascale G Charest, Muriel Aubry and Michel Bouvier
Nature Methods 1, 203–208
(December 2004); doi:10.1038/nmeth722
| Abstract | Full Text (HTML/PDF) |
news and views
Resonating to the music of ubiquitination
Sudha K Shenoy and Robert J Lefkowitz
Nature Methods 1, 191–193
(December 2004); doi:10.1038/nmeth1204-191
| Full Text (HTML/PDF) |
hypothesis
Labeled antigens and antibodies: the evolution of magic markers and magic bullets
Arthur M Silverstein
Nature Immunology 5, 1211–1217
(December 2004); doi:10.1038/ni1140
| Abstract | Full Text (HTML/PDF) |
review article
Large-scale database searching using tandem mass spectra: Looking up the answer in the back of the book
Rovshan G Sadygov, Daniel Cociorva and John R Yates III
Nature Methods 1, 195–202
(December 2004); doi:10.1038/nmeth725
| Abstract | Full Text (HTML/PDF) |
technical review
Light-activated ion channels for remote control of neuronal firing
Matthew Banghart, Katharine Borges, Ehud Isacoff, Dirk Trauner and Richard H Kramer
Nature Neuroscience 7, 1381–1386
(December 2004); doi:10.1038/nn1356
| Abstract | Full Text (HTML/PDF) |
news and views
A molecular light switch turns off neural activity
Cara Allen
Nature Neuroscience 7, 1291
(December 2004); doi:10.1038/nn1204-1291
| Full Text (HTML/PDF) |
article
High-throughput localization of functional elements by quantitative chromatin profiling
Michael O Dorschner, Michael Hawrylycz, Richard Humbert, James C Wallace, Anthony Shafer, Janelle Kawamoto, Joshua Mack, Robert Hall, Jeff Goldy, Peter J Sabo, Ajay Kohli, Qiliang Li, Michael McArthur and John A Stamatoyannopoulos
Nature Methods 1, 219–225
(December 2004); doi:10.1038/nmeth721
| Abstract | Full Text (HTML/PDF) |
November
article
RNAi living-cell microarrays for loss-of-function screens in Drosophila melanogaster cells
Douglas B Wheeler, Steve N Bailey, David A Guertin, Anne E Carpenter, Caitlin O Higgins and David M Sabatini
Nature Methods 1, 127–132
(November 2004); doi:10.1038/nmeth711
| Abstract | Full Text (HTML/PDF) |
news and views
5,000 RNAi experiments on a chip
Ben Lehner and Andrew G Fraser
Nature Methods 1, 103–104
(November 2004); doi:10.1038/nmeth1104-103
| Full Text (HTML/PDF) |
letter
Mapping the dynamic organization of the nuclear pore complex inside single living cells
Gwénaël Rabut, Valérie Doye and Jan Ellenberg
Nature Cell Biology 6, 1114–1121
(November 2004); doi:10.1038/ncb1184
| First Paragraph | Full Text (HTML/PDF) |
research article
Photoswitchable cyan fluorescent protein for protein tracking
Dmitriy M Chudakov, Vladislav V Verkhusha, Dmitry B Staroverov, Ekaterina A Souslova, Sergey Lukyanov and Konstantin A Lukyanov
Nature Biotechnology 22, 1435–1439
(December 2004); doi:10.1038/nbt1025
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news and views
Fluorescent proteins in a new light
Atsushi Miyawaki
Nature Biotechnology 22, 1374–1376
(November 2004); doi:10.1038/nbt1104-1374
| Full Text (HTML/PDF) |
news and views
Medicine: A cholesterol connection in RNAi
John J. Rossi
Nature 432, 155–156
(11 November 2004); doi:10.1038/432155a
| Full Text (HTML / PDF) |
technical report
Dissecting tumor maintenance requirements using bioluminescence imaging of cell proliferation in a mouse glioma model
Lene Uhrbom, Edward Nerio and Eric C Holland
Nature Medicine 10, 1257–1260
(November 2004); doi:10.1038/nm1120
| Abstract | Full Text (HTML/PDF) |
article
Cell-free protein production and labeling protocol for NMR-based structural proteomics
Dmitriy A Vinarov, Betsy L Lytle, Francis C Peterson, Ejan M Tyler, Brian F Volkman and John L Markley
Nature Methods 1, 149–153
(November 2004); doi:10.1038/nmeth716
| Abstract | Full Text (HTML/PDF) |
article
Identifying and quantifying in vivo methylation sites by heavy methyl SILAC
Shao-En Ong, Gerhard Mittler and Matthias Mann
Nature Methods 1, 119–126
(November 2004); doi:10.1038/nmeth715
| Abstract | Full Text (HTML/PDF) |
October
letter
Prediction of sequence-dependent and mutational effects on the aggregation of peptides and proteins
Ana-Maria Fernandez-Escamilla, Frederic Rousseau, Joost Schymkowitz and Luis Serrano
Nature Biotechnology 22, 1302–1306
(October 2004); doi:10.1038/nbt1012
| First Paragraph | Full Text (HTML/PDF) |
news and views
A universal TANGO?
Vijay S Pande
Nature Biotechnology 22, 1240–1241
(October 2004); doi:10.1038/nbt1004-1240
| Full Text (HTML/PDF) |
article
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Axel Nimmerjahn, Frank Kirchhoff, Jason N D Kerr and Fritjof Helmchen
Nature Methods 1, 31–37
(October 2004); doi:10.1038/nmeth706
| Abstract | Full Text (HTML/PDF) |
article
A custom microarray platform for analysis of microRNA gene expression
J Michael Thomson, Joel Parker, Charles M Perou and Scott M Hammond
Nature Methods 1, 47–53
(October 2004); doi:10.1038/nmeth704
| Abstract | Full Text (HTML/PDF) |
news and views
Engineering sweet targets for magic bullets
Ten Feizi
Nature Biotechnology 22, 1244–1245
(October 2004); doi:10.1038/nbt1004-1244
| Full Text (HTML/PDF) |
brief communication
A rapid method for determining protein kinase phosphorylation specificity
Jessica E Hutti, Emily T Jarrell, James D Chang, Derek W Abbott, Peter Storz, Alex Toker, Lewis C Cantley and Benjamin E Turk
Nature Methods 1, 27–29
(October 2004); doi:10.1038/nmeth708
| Abstract | Full Text (HTML/PDF) |
news and views
Novel at the library
Michael B Yaffe
Nature Methods 1, 13–14
(October 2004); doi:10.1038/nmeth1004-13
| Full Text (HTML/PDF) |
letters to nature
A relative signalling model for the formation of a topographic neural map
Michaël Reber, Patrick Burrola and Greg Lemke
Nature 431, 847–853
(14 October 2004); doi:10.1038/nature02957
| First Paragraph | Full Text (HTML / PDF) |
research article
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
Jianming Xie, Lei Wang, Ning Wu, Ansgar Brock, Glen Spraggon and Peter G Schultz
Nature Biotechnology 22, 1297–1301
(October 2004); doi:10.1038/nbt1013
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news and views
Phasing in iodine for structure determination
Zbigniew Dauter
Nature Biotechnology 22, 1239–1240
(October 2004); doi:10.1038/nbt1004-1239
| Full Text (HTML/PDF) |
article
Automated approach for quantitative analysis of complex peptide mixtures from tandem mass spectra
John D Venable, Meng-Qiu Dong, James Wohlschlegel, Andrew Dillin and John R Yates III
Nature Methods 1, 39–45
(October 2004); doi:10.1038/nmeth705
| Abstract | Full Text (HTML/PDF) |
news and views
Shotgun mass spec goes independent
Daniel C Liebler
Nature Methods 1, 16–17
(October 2004); doi:10.1038/nmeth1004-16
| Abstract | Full Text (HTML/PDF) |
news and views
Cell biology: Light on pits
Elizabeth Smythe
Nature 431, 641–642
(07 October 2004); doi:10.1038/431641a
| Full Text (HTML / PDF) |
perspectives
Innovation: Imaging gene expression in single living cells
Yaron Shav-Tal, Robert H. Singer and Xavier Darzacq
Nature Reviews Molecular Cell Biology 5, 855–862
(October 2004); doi:10.1038/nrm1494
| Abstract | Full Text (HTML / PDF) |
technical report
Volumetric computed tomography (VCT): a new technology for noninvasive, high-resolution monitoring of tumor angiogenesis
Fabian Kiessling, Susanne Greschus, Matthias P Lichy, Michael Bock, Christian Fink, Silvia Vosseler, Jens Moll, Margareta M Mueller, Norbert E. Fusenig, Horst Traupe and Wolfhard Semmler
Nature Medicine 10, 1133–1138
(October 2004); doi:10.1038/nm1101
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research article
Protein sequencing by mass analysis of polypeptide ladders after controlled protein hydrolysis
Hongying Zhong, Ying Zhang, Zhihui Wen and Liang Li
Nature Biotechnology 22, 1291–1296
(October 2004); doi:10.1038/nbt1011
| First Paragraph | Full Text (HTML/PDF) |
news and views
Climbing the protein ladder
Norman J Dovichi
Nature Biotechnology 22, 1242–1243
(October 2004); doi:10.1038/nbt1004-1242
| Full Text (HTML/PDF) |
article
In vitro selection and prediction of TIP47 protein-interaction interfaces
Alondra Schweizer Burguete, Pehr B Harbury and Suzanne R Pfeffer
Nature Methods 1, 55–60
(October 2004); doi:10.1038/nmeth702
| Abstract | Full Text (HTML/PDF) |
article
Exploiting the 21st amino acid — purifying and labeling proteins by selenolate targeting
Linda Johansson, Chunying Chen, Jan-Olov Thorell, Anna Fredriksson, Sharon Stone-Elander, Guro Gafvelin & Elias S J Arnér1
Nature Methods 1, 61–66
(October 2004); doi:10.1038/nmeth707
| Abstract | Full Text (HTML/PDF) |
technology feature
RNA interference: The silent treatment
Julie Clayton
Nature 431, 599–605
(30 September 2004); doi:10.1038/431599a
| Full Text (HTML / PDF) |
September
letters to nature
Exogenous control of mammalian gene expression through modulation of RNA self-cleavage
Laising Yen, Jennifer Svendsen, Jeng-Shin Lee, John T. Gray, Maxime Magnier, Takashi Baba, Robert J. D'Amato and Richard C. Mulligan
Nature 431, 471–476
(23 September 2004); doi:10.1038/nature02844
| First Paragraph | Full Text (HTML / PDF) |
news and views
Biological techniques: Tailor-made riboswitches
Angela K. Eggleston
Nature 431, 409
(23 September 2004); doi:10.1038/431409a
| Full Text (HTML / PDF) |
review article
The abc's (and xyz's) of peptide sequencing
Hanno Steen and Matthias Mann
Nature Reviews Molecular Cell Biology 5, 699–711
(September 2004); doi:10.1038/nrm1468
| Abstract | Full Text (HTML / PDF) |
technical report
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Miriam Bibel, Jens Richter, Katrin Schrenk, Kerry Lee Tucker, Volker Staiger, Martin Korte, Magdalena Goetz and Yves-Alain Barde
Nature Neuroscience 7, 1003–1009
(September 2004); doi:10.1038/nn1301
| Abstract | Full Text (HTML/PDF) |
research article
Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics
Blagoy Blagoev, Shao-En Ong, Irina Kratchmarova and Matthias Mann
Nature Biotechnology 22, 1139–1145
(September 2004); doi:10.1038/nbt1005
| First Paragraph | Full Text (HTML/PDF) |
news and views
Phosphoproteomics finds its timing
Sam A Johnson and Tony Hunter
Nature Biotechnology 22, 1093–1094
(September 2004); doi:10.1038/nbt0904-1093
| Full Text (HTML/PDF) |
research article
Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages
Raewyn M Seaberg, Simon R Smukler, Timothy J Kieffer, Grigori Enikolopov, Zeenat Asghar, Michael B Wheeler, Gregory Korbutt and Derek van der Kooy
Nature Biotechnology 22, 1115–1124
(September 2004); doi:10.1038/nbt1004
| Abstract | Full Text (HTML/PDF) |
news and views
β-cell precursors—a work in progress
Gordon C Weir and Susan Bonner-Weir
Nature Biotechnology 22, 1095–1096
(September 2004); doi:10.1038/nbt0904-1095
| Full Text (HTML/PDF) |
letters to nature
Direct charging of tRNACUA with pyrrolysine in vitro and in vivo
Sherry K. Blight, Ross C. Larue, Anirban Mahapatra, David G. Longstaff, Edward Chang, Gang Zhao, Patrick T. Kang, Kari B. Green-Church, Michael K. Chan and Joseph A. Krzycki
Nature 431, 333–335
(16 September 2004); doi:10.1038/nature02895
| First Paragraph | Full Text (HTML / PDF) |
news and views
Molecular biology: Genetic code seizes pyrrolysine
Paul Schimmel and Kirk Beebe
Nature 431, 257–258
(16 September 2004); doi:10.1038/431257a
| Full Text (HTML / PDF) |
technical report
Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy
Evelyn B Voura, Jyoti K Jaiswal, Hedi Mattoussi and Sanford M Simon
Nature Medicine 10, 993–998
(September 2004); doi:10.1038/nm1096
| First Paragraph | Full Text (HTML/PDF) |
perspectives
Opinion: Genetically modified mouse models for pharmacogenomic research
Stephen B. Liggett
Nature Reviews Genetics 5, 657–663
(September 2004); doi:10.1038/nrg1429
| Abstract | Full Text (HTML / PDF) |
news and views
High-throughput biochemistry heats up
F Raymond Salemme
Nature Biotechnology 22, 1100–1101
(September 2004); doi:10.1038/nbt0904-1100
| Full Text (HTML/PDF) |
research article
A simple method to cure established tumors by inflammatory killing of normal cells
Gregory A Daniels, Luis Sanchez-Perez, Rosa Maria Diaz, Timothy Kottke, Jill Thompson, Maoyi Lai, Michael Gough, Mahzuz Karim, Andrew Bushell, Heung Chong, Alan Melcher, Kevin Harrington and Richard G Vile
Nature Biotechnology 22, 1125–1132
(September 2004); doi:10.1038/nbt1007
| Abstract | Full Text (HTML/PDF) |
news and views
Immunotherapy dispenses with tumor antigens
Soldano Ferrone
Nature Biotechnology 22, 1096–1098
(September 2004); doi:10.1038/nbt0904-1096
| Full Text (HTML/PDF) |
research article
Rapid and ultra–sensitive determination of enzyme activities using surface–enhanced resonance Raman scattering
Barry D Moore, Lorna Stevenson, Alan Watt, Sabine Flitsch, Nicolas J Turner, Chris Cassidy and Duncan Graham
Nature Biotechnology 22, 1133–1138
(September 2004); doi:10.1038/nbt1003
| Abstract | Full Text (HTML/PDF) |
news and views
Finding enzymatic gold on silver surfaces
Uwe T Bornscheuer
Nature Biotechnology 22, 1098–1099
(September 2004); doi:10.1038/nbt0904–1098
| Full Text (HTML/PDF) |
August
news and views
Chemical biology: Green fluorescent RNA
Michael Famulok
Nature 430, 976–977
(26 August 2004); doi:10.1038/430976a
| Full Text (HTML / PDF) |
perspectives
Innovation: Seventeen-colour flow cytometry: unravelling the immune system
Stephen P. Perfetto, Pratip K. Chattopadhyay and Mario Roederer
Nature Reviews Immunology 4, 648–655
(August 2004); doi:10.1038/nri1416
| Summary | Full Text (HTML / PDF) |
research article
In vivo cancer targeting and imaging with semiconductor quantum dots
Xiaohu Gao, Yuanyuan Cui, Richard M Levenson, Leland W K Chung and Shuming Nie
Nature Biotechnology 22, 969–976
(August 2004); doi:10.1038/nbt994
| Abstract | Full Text (HTML/PDF) |
news and views
Zooming in and out with quantum dots
Rakesh K Jain and Mark Stroh
Nature Biotechnology 22, 959–960
(August 2004); doi:10.1038/nbt0804-959
| Full Text (HTML/PDF) |
letters to nature
Chemical remodelling of cell surfaces in living animals
Jennifer A. Prescher, Danielle H. Dube and Carolyn R. Bertozzi
Nature 430, 873–877
(19 August 2004); doi:10.1038/nature02791
| First Paragraph | Full Text (HTML / PDF) |
news and views
Chemical biology: Hitting the sweet spot
David A. Tirrell
Nature 430, 837
(19 August 2004); doi:10.1038/430837a
| Full Text (HTML / PDF) |
letter
Mutagenic Insertion and Chromosome Engineering Resource (MICER)
David J Adams, Patrick J Biggs, Tony Cox, Rob Davies, Louise van der Weyden, Jos Jonkers, James Smith, Bob Plumb, Ruth Taylor, Ichiko Nishijima, Yuejin Yu, Jane Rogers and Allan Bradley
Nature Genetics 36, 867–871
(August 2004); doi:10.1038/ng1388
| First Paragraph | Full Text (HTML/PDF) |
research article
Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture
Eberhard Durr, Jingyi Yu, Karolina M Krasinska, Lucy A Carver, John R Yates, Jacqueline E Testa III, Phil Oh and Jan E Schnitzer
Nature Biotechnology 22, 985–992
(August 2004); doi:10.1038/nbt993
| Abstract | Full Text (HTML/PDF) |
research article
Polyvalent dendrimer glucosamine conjugates prevent scar tissue formation
Sunil Shaunak, Sharyn Thomas, Elisabetta Gianasi, Antony Godwin, Emma Jones, Ian Teo, Kamiar Mireskandari, Philip Luthert, Ruth Duncan, Steve Patterson, Peng Khaw and Steve Brocchini
Nature Biotechnology 22, 977–984
(August 2004); doi:10.1038/nbt995
| Abstract | Full Text (HTML/PDF) |
research article
In silico simulation of biological network dynamics
Lukasz Salwinski and David Eisenberg
Nature Biotechnology 22, 1017–1019
(August 2004); doi:10.1038/nbt991
| First Paragraph | Full Text (HTML/PDF) |
news and views
The need for speed in stochastic simulation
Larry Lok
Nature Biotechnology 22, 964–965
(August 2004); doi:10.1038/nbt0804-964
| Full Text (HTML/PDF) |
perspectives
Opinion: Integrated global profiling of cancer
Samir Hanash
Nature Reviews Cancer 4, 638–644
(August 2004); doi:10.1038/nrc1414
| Abstract | Full Text (HTML / PDF) |
July
research article
An engineered epigenetic transgene switch in mammalian cells
Beat P Kramer, Alessandro Usseglio Viretta, Marie Daoud-El Baba, Dominique Aubel, Wilfried Weber and Martin Fussenegger
Nature Biotechnology 22, 867–870
(July 2004); doi:10.1038/nbt980
| First Paragraph | Full Text (HTML/PDF) |
research article
A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications
Dawei Guo, Tony R Hazbun, Xin-Jing Xu, Sze-Ling Ng, Stanley Fields and Min-Hao Kuo
Nature Biotechnology 22, 888–892
(July 2004); doi:10.1038/nbt985
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research article
Temperature-sensitive control of protein activity by conditionally splicing inteins
Martin P Zeidler, Change Tan, Yohanns Bellaiche, Sara Cherry, Sabine Häder, Urte Gayko and Norbert Perrimon
Nature Biotechnology 22, 871–876
(July 2004); doi:10.1038/nbt979
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news and views
The ins and outs of gene expression control
Francine B Perler
Nature Biotechnology 22, 824–826
(July 2004); doi:10.1038/nbt0704-824
| Full Text (HTML/PDF) |
research article
Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells
Daniel G Anderson, Shulamit Levenberg and Robert Langer
Nature Biotechnology 22, 863–866
(July 2004); doi:10.1038/nbt981
| First Paragraph | Full Text (HTML/PDF) |
news and views
Biomaterials science and high-throughput screening
Jeffrey A Hubbell
Nature Biotechnology 22, 828–829
(July 2004); doi:10.1038/nbt0704-828
| Full Text (HTML/PDF) |
news and views feature
The RNAi revolution
Carl D. Novina and Phillip A. Sharp
Nature 430, 161–164
(08 July 2004); doi:10.1038/430161a
| Full Text (HTML / PDF) |
research article
Engineered riboregulators enable post-transcriptional control of gene expression
Farren J Isaacs, Daniel J Dwyer, Chunming Ding, Dmitri D Pervouchine, Charles R Cantor and James J Collins
Nature Biotechnology 22, 841–847
(July 2004); doi:10.1038/nbt986
| Abstract | Full Text (HTML/PDF) |
news and views
Custom design of metabolism
James C Liao
Nature Biotechnology 22, 823–824
(July 2004); doi:10.1038/nbt0704-823
| Full Text (HTML/PDF) |
letter
Cold-shock induced high-yield protein production in Escherichia coli
Guoliang Qing, Li-Chung Ma, Ahmad Khorchid, G V T Swapna, Tapas K Mal, Masanori Mitta Takayama, Bing Xia, Sangita Phadtare, Haiping Ke, Thomas Acton, Gaetano T Montelione, Mitsuhiko Ikura and Masayori Inouye
Nature Biotechnology 22, 877–882
(July 2004); doi:10.1038/nbt984
| First Paragraph | Full Text (HTML/PDF) |
news and views
A cool way to make proteins
Catherine H Schein
Nature Biotechnology 22, 826–827
(July 2004); doi:10.1038/nbt0704-826
| Full Text (HTML/PDF) |
June
technical report
A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients
William J Rosoff, Jeffrey S Urbach, Mark A Esrick, Ryan G McAllister, Linda J Richards and Geoffrey J Goodhill
Nature Neuroscience 7, 678–682
(June 2004); doi:10.1038/nn1259
| Abstract | Full Text (HTML/PDF) |
news and views
Assaying axon sensitivity
Kalyani Narasimhan
Nature Neuroscience 7, 574
(June 2004); doi:10.1038/nn0604-574
| Full Text (HTML/PDF) |
research article
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues
Gerold Schmitt-Ulms, Kirk Hansen, Jialing Liu, Cynthia Cowdrey, Jian Yang, Stephen J DeArmond, Fred E Cohen, Stanley B Prusiner and Michael A Baldwin
Nature Biotechnology 22, 724–731
(June 2004); doi:10.1038/nbt969
| Abstract | Full Text (HTML/PDF) |
technical report
Bioluminescent imaging of Cdk2 inhibition in vivo
Guo-Jun Zhang, Michal Safran, Wenyi Wei, Erik Sorensen, Peter Lassota, Nikolai Zhelev, Donna S Neuberg, Geoffrey Shapiro, and William G Kaelin Jr
Nature Medicine 10, 643–648
(June 2004); doi:10.1038/nm1047
| Abstract | Full Text (HTML/PDF) |
news and views
Targeting tumors: seeing the light
Charlotte Schubert
Nature Medicine 10, 580
(June 2004); doi:10.1038/nm0604-580
| Full Text (HTML/PDF) |
research article
Chemical screening by mass spectrometry to identify inhibitors of anthrax lethal factor
Dal-Hee Min, Wei-Jen Tang and Milan Mrksich
Nature Biotechnology 22, 717–723
(June 2004); doi:10.1038/nbt973
| Abstract | Full Text (HTML/PDF) |
research article
Imaging the pharmacodynamics of HER2 degradation in response to Hsp90 inhibitors
Peter M Smith-Jones, David B Solit, Timothy Akhurst, Farzana Afroze, Neal Rosen and Steven M Larson
Nature Biotechnology 22, 701–706
(June 2004); doi:10.1038/nbt968
| Abstract | Full Text (HTML/PDF) |
news and views
An image worth a thousand lives?
Lisa A Hammond, Sean X Cavanaugh and Charles R Thomas Jr
Nature Biotechnology 22, 677–678
(June 2004); doi:10.1038/nbt0604-677
| Full Text (HTML/PDF) |
May
letters to nature
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain
Jin-Biao Ma, Keqiong Ye and Dinshaw J. Patel
Nature 429, 318–322
(20 May 2004); doi:10.1038/nature02519
| First Paragraph | Full Text (HTML / PDF) |
article
T cell killing does not require the formation of a stable mature immunological synapse
Marco A Purbhoo, Darrell J Irvine, Johannes B Huppa and Mark M Davis
Nature Immunology 5, 524–530
(May 2004); doi:10.1038/ni1058
| Abstract | Full Text (HTML/PDF) |
compbio
Microarray reality checks in the context of a complex disease
George L Gabor Miklos and Ryszard Maleszka
Nature Biotechnology 22, 615–621
(May 2004); doi:10.1038/nbt965
| Abstract | Full Text (HTML/PDF) |
news and views
Encoded self-assembly chemical libraries
Samu Melkko, Jörg Scheuermann, Christoph E Dumelin & Dario Neri
Nature Biotechnology 7, 568–574
(May 2004); doi:10.1038/nbt961
| Full Text (HTML/PDF) |
perspectives
Innovation: Novel ChIP-based strategies to uncover transcription factor target genes in the immune system
Amy S. Weinmann
Nature Reviews Immunology 4, 381–386
(May 2004); doi:10.1038/nri1353
| Abstract | Full Text (HTML / PDF) |
review article
Improving the efficiency of RNA interference in mammals
Vivek Mittal
Nature Reviews Genetics 5, 355–365
(May 2004); doi:10.1038/nrg1323
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research article
High-affinity binders selected from designed ankyrin repeat protein libraries
H Kaspar Binz, Patrick Amstutz, Andreas Kohl, Michael T Stumpp, Christophe Briand, Patrik Forrer, Markus G Grütter and Andreas Plückthun
Nature Biotechnology 22, 575–582
(May 2004); doi:10.1038/nbt962
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Next-generation protein drugs
Ian M Tomlinson
Nature Biotechnology 22, 521–522
(May 2004); doi:10.1038/nbt0504-521
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April
news and views
A plethora of sites
Ghia Euskirchen and Michael Snyder
Nature Genetics 36, 325–326
(April 2004); doi:10.1038/ng0404-325
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research article
Reversible site-selective labeling of membrane proteins in live cells
Emmanuel G Guignet, Ruud Hovius and Horst Vogel
Nature Biotechnology 22, 440–444
(April 2004); doi:10.1038/nbt954
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news and views
Finding needles in haystacks
Jonathan A Javitch
Nature Biotechnology 22, 394–396
(April 2004); doi:10.1038/nbt0404-394
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letters to nature
Programmed population control by cell-cell communication and regulated killing
Lingchong You, Robert Sidney Cox III, Ron Weiss and Frances H. Arnold
Nature 428, 868–871
(22 April 2004); doi:10.1038/nature02491
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research article
Differential quantitative analysis of MHC ligands by mass spectrometry using stable isotope labeling
Claudia Lemmel, Steffen Weik, Ute Eberle, Jörn Dengjel, Thomas Kratt, Horst-Dieter Becker, Hans-Georg Rammensee and Stefan Stevanovic;
Nature Biotechnology 22, 450–454
(April 2004); doi:10.1038/nbt947
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review article
siRNAs: applications in functional genomics and potential as therapeutics
Yair Dorsett and Thomas Tuschl
Nature Reviews Drug Discovery 3, 318–329
(April 2004); doi:10.1038/nrd1345
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review article
Neural activity and the dynamics of central nervous system development
Jackie Yuanyuan Hua and Stephen J Smith
Nature Neuroscience 7, 327–332
(April 2004); doi:10.1038/nn1218
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review article
Small-molecule inhibitors of protein-protein interactions: progressing towards the dream
Michelle R. Arkin and James A. Wells
Nature Reviews Drug Discovery 3, 301–317
(April 2004); doi:10.1038/nrd1343
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Unshackling expression profiling arrays
Darrell P Chandler
Nature Biotechnology 22, 396–397
(April 2004); doi:10.1038/nbt0404-396
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research article
An improved cyan fluorescent protein variant useful for FRET
Mark A Rizzo, Gerald H Springer, Butch Granada and David W Piston
Nature Biotechnology 22, 445–449
(April 2004); doi:10.1038/nbt945
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review article
Designing materials for biology and medicine
Robert Langer and David A. Tirrell
Nature 428, 487–492
(01 April 2004); doi:10.1038/nature02388
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Cancer's sphere of influence
Charlotte Schubert
Nature Medicine 10, 349
(April 2004); doi:10.1038/nm0404-349
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research article
Expression profiling using a hexamer-based universal microarray
Matthew E Roth, Li Feng, Kevin J McConnell, Paul J Schaffer, Cesar E Guerra, Jason P Affourtit, Kevin R Piper, Lorri Guccione, Jayashree Hariharan, Maura J Ford, Stephen W Powell, Harish Krishnaswamy, Jennifer Lane, Lisa Guccione, Gino Intrieri, Jane S Merkel, Clotilde Perbost, Anthony Valerio, Brenda Zolla, Carol D Graham, Jonathan Hnath, Chris Michaelson, Rixin Wang, Baoge Ying, Conrad Halling, Craig E Parman, Debasish Raha, Brent Orr, Barbara Jedrzkiewicz, Ji Liao, Anton Tevelev, Martin J Mattessich, David M Kranz, Michelle Lacey, Joseph C Kaufman, Junhyong Kim, Darin R Latimer and Paul M Lizardi
Nature Biotechnology 22, 418–426
(April 2004); doi:10.1038/nbt948
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March
letters to nature
A resource for large-scale RNA-interference-based screens in mammals
Patrick J. Paddison, Jose M. Silva, Douglas S. Conklin , Mike Schlabach, Mamie Li, Shola Aruleba, Vivekanand Balija, Andy O'Shaughnessy, Lidia Gnoj, Kim Scobie, Kenneth Chang, Thomas Westbrook, Michele Cleary, Ravi Sachidanandam, W. Richard McCombie, Stephen J. Elledge and Gregory J. Hannon
Nature 428, 427–431
(25 March 2004); doi:10.1038/nature02370
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letters to nature
A large-scale RNAi screen in human cells identifies new components of the p53 pathway
Katrien Berns, E. Marielle Hijmans, Jasper Mullenders, Thijn R. Brummelkamp, Arno Velds, Mike Heimerikx, Ron M. Kerkhoven, Mandy Madiredjo, Wouter Nijkamp, Britta Weigelt, Reuven Agami, Wei Ge, Guy Cavet, Peter S. Linsley, Roderick L. Beijersbergen and René Bernards
Nature 428, 431–437
(25 March 2004); doi:10.1038/nature02371
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news and views
RNA interference: Human genes hit the big screen
Andrew Fraser
Nature 428, 375–378
(25 March 2004); doi:10.1038/428375a
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letter
Gene expression-based high-throughput screening(GE-HTS) and application to leukemia differentiation
Kimberly Stegmaier, Kenneth N Ross, Sierra A Colavito, Shawn O'Malley, Brent R Stockwell and Todd R Golub
Nature Genetics 36, 257–263
(March 2004); doi:10.1038/ng1305
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Gene expression as a drug discovery tool
William E Evans and R Kip Guy
Nature Genetics 36, 214–215
(March 2004); doi:10.1038/ng0304-214
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research article
Rational siRNA design for RNA interference
Angela Reynolds, Devin Leake, Queta Boese, Stephen Scaringe, William S Marshall and Anastasia Khvorova
Nature Biotechnology 22, 326–330
(March 2004); doi:10.1038/nbt936
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research article
Antibody-targeted cell fusion
Takafumi Nakamura, Kah-Whye Peng, Sompong Vongpunsawad, Mary Harvey, Hiroyuki Mizuguchi, Takao Hayakawa, Roberto Cattaneo and Stephen J Russell
Nature Biotechnology 22, 331–336
(March 2004); doi:10.1038/nbt942
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review article
Ontologies in biology: design, applications and future challenges
Jonathan B. L. Bard and Seung Y. Rhee
Nature Reviews Genetics 5, 213–222
(March 2004); doi:10.1038/nrg1295
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research article
Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase
Dong-Ho Kim, Michael Longo, Young Han, Patric Lundberg, Edouard Cantin and John J Rossi
Nature Biotechnology 22, 321–325
(March 2004); doi:10.1038/nbt940
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news and views
Knockdown by RNAi—proceed with caution
Charles E Samuel
Nature Biotechnology 22, 280–282
(March 2004); doi:10.1038/nbt0304-280
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perspectives
Guidelines: Expression profiling — best practices for data generation and interpretation in clinical trials
The Tumor Analysis Best Practices Working Group
Nature Reviews Genetics 5, 229–237
(March 2004); doi:10.1038/nrg1297
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article
In vivo imaging of synapse formation on a growing dendritic arbor
Cristopher M Niell, Martin P Meyer and Stephen J Smith
Nature Neuroscience 7, 254–260
(March 2004); doi:10.1038/nn1191
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Single dendrite seeks stable relationship
Josh Morgan and Rachel Wong
Nature Neuroscience 7, 205–206
(March 2004); doi:10.1038/nn0304-205
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research article
Probing the precision of the mitotic clock with a live-cell fluorescent biosensor
Joshua T Jones, Jason W Myers, James E Ferrell and Jr Tobias Meyer
Nature Biotechnology 22, 306–312
(March 2004); doi:10.1038/nbt941
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The pulse of mitosis
Conly L Rieder and Alexei Mikhailov
Nature Biotechnology 22, 279–280
(March 2004); doi:10.1038/nbt0304-279
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letter
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac
Stephen T Thibault, Matthew A Singer, Wesley Y Miyazaki, Brett Milash, Nicholas A Dompe, Carol M Singh, Ross Buchholz, Madelyn Demsky, Robert Fawcett, Helen L Francis-Lang, Lisa Ryner, Lai Man Cheung, Angela Chong, Cathy Erickson, William W Fisher, Kimberly Greer, Stephanie R Hartouni, Elizabeth Howie, Lakshmi Jakkula, Daniel Joo, Keith Killpack, Alex Laufer, Julie Mazzotta, Ronald D Smith, Lynn M Stevens, Christiana Stuber, Lory R Tan, Richard Ventura, Alesa Woo, Irena Zakrajsek, Lora Zhao, Feng Chen, Candace Swimmer, Casey Kopczynski, Geoffrey Duyk, Margaret L Winberg and Jonathan Margolis
Nature Genetics 36, 283–287
(March 2004); doi:10.1038/ng1314
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letter
Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome
Annette L Parks, Kevin R Cook, Marcia Belvin, Nicholas A Dompe, Robert Fawcett, Kari Huppert, Lory R Tan, Christopher G Winter, Kevin P Bogart, Jennifer E Deal, Megan E Deal-Herr, Deanna Grant, Marie Marcinko, Wesley Y Miyazaki, Stephanie Robertson, Kenneth J Shaw, Mariano Tabios, Valentina Vysotskaia, Lora Zhao, Rachel S Andrade, Kyle A Edgar, Elizabeth Howie, Keith Killpack, Brett Milash, Amanda Norton, Doua Thao, Kellie Whittaker, Millicent A Winner, Lori Friedman, Jonathan Margolis, Matthew A Singer, Casey Kopczynski, Daniel Curtis, Thomas C Kaufman, Gregory D Plowman, Geoffrey Duyk and Helen L Francis-Lang
Nature Genetics 36, 288–292
(March 2004); doi:10.1038/ng1312
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Flying in the face of total disruption
P Robin Hiesinger and Hugo J Bellen
Nature Genetics 36, 211–212
(March 2004); doi:10.1038/ng0304-211
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February
research article
The HUPO PSI's Molecular Interaction format—a community standard for the representation of protein interaction data
Henning Hermjakob, Luisa Montecchi-Palazzi, Gary Bader, Jérôme Wojcik, Lukasz Salwinski, Arnaud Ceol, Susan Moore, Sandra Orchard, Ugis Sarkans, Christian von Mering, Bernd Roechert, Sylvain Poux, Eva Jung, Henning Mersch, Paul Kersey, Michael Lappe, Yixue Li, Rong Zeng, Debashis Rana, Macha Nikolski, Holger Husi, Christine Brun, K Shanker, Seth G N Grant, Chris Sander, Peer Bork, Weimin Zhu, Akhilesh Pandey, Alvis Brazma, Bernard Jacq, Marc Vidal, David Sherman, Pierre Legrain, Gianni Cesareni, Ioannis Xenarios, David Eisenberg, Boris Steipe, Chris Hogue and Rolf Apweiler
Nature Biotechnology 22, 177–183
(February 2004); doi:10.1038/nbt926
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letters to nature
Multistability in the lactose utilization network of Escherichia coli
Ertugrul M. Ozbudak, Mukund Thattai, Han N. Lim, Boris I. Shraiman and Alexander van Oudenaarden
Nature 427, 737–740
(19 February 2004); doi:10.1038/nature02298
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perspectives
Innovation: TILLING — a high-throughput harvest for functional genomics
Derek L. Stemple
Nature Reviews Genetics 5, 145–150
(February 2004); doi:10.1038/nrg1273
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Label-free detection becomes crystal clear
Paul S Cremer
Nature Biotechnology 22, 172–173
(February 2004); doi:10.1038/nbt0204-172
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review article
Network biology: understanding the cell's functional organization
Albert-László Barabási and Zoltán N. Oltvai
Nature Reviews Genetics 5, 101–113
(February 2004); doi:10.1038/nrg1272
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article
Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction
Diane S Lidke, Peter Nagy, Rainer Heintzmann, Donna J Arndt-Jovin, Janine N Post, Hernan E Grecco, Elizabeth A Jares-Erijman and Thomas M Jovin
Nature Biotechnology 22, 198–203
(February 2004); doi:10.1038/nbt929
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Enlightened receptor dynamics
Gal Gur and Yosef Yarden
Nature Biotechnology 22, 169–170
(February 2004); doi:10.1038/nbt0204-169
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review article
cAMP detection methods in HTS: selecting the best from the rest
Christine Williams
Nature Reviews Drug Discovery 3, 125–135
(February 2004); doi:10.1038/nrd1306
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article
A physical and functional map of the human TNF-α/NF-kB signal transduction pathway
Tewis Bouwmeester, Angela Bauch, Heinz Ruffner, Pierre-Olivier Angrand, Giovanna Bergamini, Karen Croughton, Cristina Cruciat, Dirk Eberhard, Julien Gagneur, Sonja Ghidelli, Carsten Hopf, Bettina Huhse, Raffaella Mangano, Anne-Marie Michon, Markus Schirle, Judith Schlegl, Markus Schwab, Martin A. Stein, Andreas Bauer, Georg Casari, Gerard Drewes, Anne-Claude Gavin, David B. Jackson, Gerard Joberty, Gitte Neubauer, Jens Rick, Bernhard Kuster and Giulio Superti-Furga
Nature Cell Biology 6, 97–105
(February 2004); doi:10.1038/ncb1086
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Keeping cartographers busy
Matthew S. Hayden and Sankar Ghosh
Nature Cell Biology 6, 87–89
(February 2004); doi:10.1038/ncb0204-87
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letter
Intensity-based protein identification by machine learning from a library of tandem mass spectra
Joshua E Elias, Francis D Gibbons, Oliver D King, Frederick P Roth and Steven P Gygi
Nature Biotechnology 22, 214–219
(February 2004); doi:10.1038/nbt930
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letter
FRET between cardiac Na+ channel subunits measured with a confocal microscope and a streak camera
Christoph Biskup, Thomas Zimmer and Klaus Benndorf
Nature Biotechnology 22, 220–224
(February 2004) ; doi:10.1038/nbt935
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January
article
Summing up the noise in gene networks
Johan Paulsson
Nature 427, 415–418
(29 January 2004); doi:10.1038/nature02257
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technical report
A transgenic mouse model for monitoring endoplasmic reticulum stress
Takao Iwawaki, Ryoko Akai, Kenji Kohno and Masayuki Miura
Nature Medicine 10, 98–102
(January 2004); doi:10.1038/nm970
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article
Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways
Ainslie B Parsons, Renée L Brost, Huiming Ding, Zhijian Li, Chaoying Zhang, Bilal Sheikh, Grant W Brown, Patricia M Kane, Timothy R Hughes & Charles Boone
Nature Biotechnology 22, 62–69
(January 2004); doi:10.1038/nbt919
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The biological magic behind the bullets
Brent R Stockwell
Nature Biotechnology 22, 37–38
(January 2004); doi:10.1038/nbt0104-37
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review article
Fluorescent probes for proteolysis: Tools for drug discovery
Jacques Neefjes and Nico P. Dantuma
Nature Reviews Drug Discovery 3, 58–69
(January 2004); doi:10.1038/nrd1282
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letter
Unraveling protein interaction networks with near-optimal efficiency
Michael Lappe & Liisa Holm
Nature Structural Biology 22, 98–103
(January 2004); doi:10.1038/nbt921
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perspective
The use of nanocrystals in biological detection
Paul Alivisatos
Nature Biotechnology 22, 47–52
(January 2004); doi:10.1038/nbt927
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article
Gaining confidence in high-throughput protein interaction networks
Joel S Bader, Amitabha Chaudhuri, Jonathan M Rothberg & John Chant
Nature Biotechnology 22, 78–85
(14 December 2003); doi:10.1038/nbt924
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Protein interaction maps on the fly
Peter Uetz & Michael J Pankratz
Nature Biotechnology 22, 43–44
(January 2004); doi:10.1038/nbt0104-43
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