Research library: Techniques
2006:
December
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December
article
Monitoring regulated protein-protein interactions using split TEV
Michael C Wehr, Rico Laage, Ulrike Bolz, Tobias M Fischer, Sylvia Grünewald, Sigrid Scheek, Alfred Bach, Klaus-Armin Nave & Moritz J Rossner
Nature Methods 3, 1128–1134
(December 2006); doi:10.1038/nmeth967
| Summary | Full Text (HTML / PDF) |
news and views
Complementary methods for studies of protein interactions in living cells
Tom K Kerppola
Nature Methods 3, 969–971
(December 2006); doi:10.1038/nmeth1206-969
| Summary | Full Text (HTML / PDF) |
article
Two-chamber AFM: probing membrane proteins separating two aqueous compartments
Rui Pedro Gonçalves, Guillaume Agnus, Pierre Sens, Christine Houssin, Bernard Bartenlian & Simon Scheuring
Nature Methods 3, 1007–1012
(December 2006); doi:10.1038/nmeth965
| Summary | Full Text (HTML / PDF) |
article
Atomic force microscopy of membrane proteins separating two aqueous compartments
Yves F Dufrêne
Nature Methods 3, 973–975
(December 2006); doi:10.1038/nmeth1206-973
| Summary | Full Text (HTML / PDF) |
article
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
Tilmann Bürckstümmer, Keiryn L Bennett, Adrijana Preradovic, Gregor Schütze, Oliver Hantschel, Giulio Superti-Furga & Angela Bauch
Nature Methods 3, 1013–1019
(December 2006); doi:10.1038/nmeth968
| Summary | Full Text (HTML / PDF) |
brief communication
Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library
Marco A Mena, Thomas P Treynor, Stephen L Mayo & Patrick S Daugherty
Nature Biotechnology 24, 1569–1571
(December 2006); doi:10.1038/nbt1264
| Summary | Full Text (HTML / PDF) |
article
Direct observation of individual endogenous protein complexes in situ by proximity ligation
Ola Söderberg, Mats Gullberg, Malin Jarvius, Karin Ridderstråle, Karl-Johan Leuchowius, Jonas Jarvius, Kenneth Wester, Per Hydbring, Fuad Bahram, Lars-Gunnar Larsson & Ulf Landegren
Nature Methods 3, 995–1000
(December 2006); doi:10.1038/nrd2154
| Summary | Full Text (HTML / PDF) |
news and views
Single protein complex visualization: Seeing is believing
Sam Lievens & Jan Tavernier
Nature Methods 3, 971–972
(December 2006); doi:10.1038/nmeth1206-971
| Summary | Full Text (HTML / PDF) |
article
A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer
John R James, Marta I Oliveira, Alexandre M Carmo, Andrea Iaboni & Simon J Davis
Nature Methods 3, 1001–1006
(December 2006); doi:10.1038/nmeth978
| Summary | Full Text (HTML / PDF) |
news and views
G protein—coupled receptors: too many dimers?
Martin J Lohse
Nature Methods 3, 972–973
(December 2006); doi:10.1038/nmeth1206-972
| Summary | Full Text (HTML / PDF) |
November
article
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
Tilmann Bürckstümmer, Keiryn L Bennett, Adrijana Preradovic, Gregor Schütze, Oliver Hantschel, Giulio Superti-Furga & Angela Bauch
Nature Methods 3, 1013–1019
(November 2006); doi:10.1038/nmeth968
| Summary | Full Text (HTML / PDF) |
letter
Visualization of transient encounter complexes in protein-protein association
Chun Tang, Junji Iwahara and G. Marius Clore
Nature 444, 383–386
(16 November 2006); doi:10.1038/nature05201
| First Paragraph | Full Text (HTML / PDF) |
article
A new strategy for structure determination of large proteins in solution without deuteration
Yingqi Xu, Yu Zheng, Jing-Song Fan & Daiwen Yang
Nature Methods 3, 931–937
(November 2006); doi:10.1038/nmeth938
| Summary | Full Text (HTML / PDF) |
October
perspective
Channelrhodopsin-2 and optical control of excitable cells
Feng Zhang, Li-Ping Wang, Edward S Boyden & Karl Deisseroth
Nature Methods 5, 785–792
(October 2006); doi:10.1038/nmeth936
| Summary | Full Text (HTML / PDF) |
article
State-based discovery: a multidimensional screen for small-molecule modulators of EGF signaling
Mark Sevecka & Gavin MacBeath
Nature Methods 5, 825–831
(October 2006); doi:10.1038/nmeth931
| Summary | Full Text (HTML / PDF) |
article
Analyzing proteome topology and function by automated multidimensional fluorescence microscopy
Walter Schubert, Bernd Bonnekoh, Ansgar J Pommer, Lars Philipsen, Raik Böckelmann, Yanina Malykh, Harald Gollnick, Manuela Friedenberger, Marcus Bode & Andreas W M Dress
Nature Biotechnology 24, 1270–1278
(2006); doi:10.1038/nbt1250
| Summary | Full Text (HTML / PDF) |
news and views
Putting proteins on the map
Robert F Murphy
Nature Biotechnology 24, 1223–1224
(2006); doi:10.1038/nbt1006-1223
| Summary | Full Text (HTML / PDF) |
commentary
Minimizing the risk of reporting false positives in large-scale RNAi screens
Christophe J Echeverri, Philip A Beachy, Buzz Baum, Michael Boutros, Frank Buchholz, Sumit K Chanda, Julian Downward, Jan Ellenberg, Andrew G Fraser, Nir Hacohen, William C Hahn, Aimee L Jackson, Amy Kiger, Peter S Linsley, Lawrence Lum, Yong Ma, Bernard Mathey-Prévôt, David E Root, David M Sabatini, Jussi Taipale, Norbert Perrimon & René Bernards
Nature Methods 3, 777–779
(2006); doi:10.1038/nmeth1006-777
| Summary | Full Text (HTML / PDF) |
perspective
Channelrhodopsin-2 and optical control of excitable cells
Feng Zhang, Li-Ping Wang, Edward S Boyden & Karl Deisseroth
Nature Methods 3, 785–792
(October 2006); doi:10.1038/nmeth936
| Summary | Full Text (HTML / PDF) |
September
perspective
High-throughput fluorescence microscopy for systems biology
Rainer Pepperkok and Jan Ellenberg
Nature Reviews Molecular Cell Biology 7, 690–696
(September 2006); doi:10.1038/nrm1979
| Summary | Full Text (HTML / PDF) |
letter
Prevalence of off-target effects in Drosophila RNA interference screens
Yong Ma, Adrian Creanga, Lawrence Lum and Philip A. Beachy
Nature 443, 359–363
(21 September 2006); doi:10.1038/nature05179
| Summary | Full Text (HTML / PDF) |
article
Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domains
Kevin Dierck, Kazuya Machida, Anja Voigt, Julian Thimm, Martin Horstmann, Walter Fiedler, Bruce J Mayer & Peter Nollau
Nature Methods 3, 737–744
(23 August 2006); doi:10.1038/nmeth917
| Summary | Full Text (HTML / PDF) |
August
perspective
Proteome chips for whole-organism assays
Li A. Kung and Michael Snyder
Nature Reviews Molecular Cell Biology 7, 617–622
(August 2006); doi:10.1038/nrm1941
| Summary | Full Text (HTML / PDF) |
review
Chemical labeling strategies for cell biology
Kevin M Marks & Garry P Nolan
Nature Methods 12, 591–596
(August 2006); doi:10.1038/nmeth906
| Summary | Full Text (HTML / PDF) |
reviews
Standards for systems biology
Alvis Brazma, Maria Krestyaninova and Ugis Sarkans
Nature Review Genetics 7, 593–605
(August 2006); doi:10.1038/nrg1922
| Summary | Full Text (HTML / PDF) |
article
Dissecting self-renewal in stem cells with RNA interference
Natalia Ivanova, Radu Dobrin, Rong Lu, Iulia Kotenko, John Levorse, Christina DeCoste, Xenia Schafer, Yi Lun and Ihor R. Lemischka
Nature 442, 533–538
(02 August 2006); doi:10.1038/nature04915
| First Paragraph | Full Text (HTML / PDF) |
article
A comprehensive library of fluorescent transcriptional reporters for Escherichia coli
Alon Zaslaver, Anat Bren, Michal Ronen, Shalev Itzkovitz, Ilya Kikoin, Seagull Shavit, Wolfram Liebermeister, Michael G Surette & Uri Alon
Nature Methods 3, 623–638
(August 2006); doi:10.1038/nmeth895
| Summary | Full Text (HTML / PDF) |
article
High-throughput screening methodology for the directed evolution of glycosyltransferases
Amir Aharoni, Karena Thieme, Cecilia P C Chiu, Sabrina Buchini, Luke L Lairson, Hongming Chen, Natalie C J Strynadka, Warren W Wakarchuk & Stephen G Withers
Nature Methods 3, 609–614
(August 2006); doi:10.1038/nmeth899
| Summary | Full Text (HTML / PDF) |
July
letter
Endothelial tubes assemble from intracellular vacuoles in vivo
Makoto Kamei, W. Brian Saunders, Kayla J. Bayless, Louis Dye, George E. Davis and Brant M. Weinstein
Nature 442, 453–456
(27 July 2006); doi:10.1038/nature04923
| First Paragraph | Full Text (HTML / PDF) |
news and views
Developmental biology: The hole picture
Keith Mostov and Fernando Martin-Belmonte
Nature 442, 363–364
(27 July 2006); doi:10.1038/442363a
| Full Text (HTML / PDF) |
technology feature
Microarrays: branching out from expression analysis
Laura Bonetta
Nature Methods 3, 571–578
(July 2006); doi:10.1038/nmeth0706-571
| Summary | Full Text (HTML / PDF) |
letter
Atom-by-atom analysis of global downhill protein folding
Mourad Sadqi, David Fushman and Victor Muñoz
Nature 442, 317–321
(20 July 2006); doi:10.1038/nature04859
| First Paragraph | Full Text (HTML / PDF) |
article
Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins
Alex Sigal, Ron Milo, Ariel Cohen, Naama Geva-Zatorsky, Yael Klein, Inbal Alaluf, Naamah Swerdlin, Natalie Perzov, Tamar Danon, Yuvalal Liron, Tal Raveh, Anne E Carpenter, Galit Lahav & Uri Alon
Nature Methods 7, 525–531
(July 2006); doi:10.1038/nmeth892
| Summary | Full Text (HTML / PDF) |
article
Automated identification of SUMOylation sites using mass spectrometry and SUMmOn pattern recognition software
Patrick G A Pedrioli, Brian Raught, Xiang-Dong Zhang, Richard Rogers, John Aitchison, Michael Matunis & Ruedi Aebersold
Nature Methods 3, 533–539
(21 June 2006); doi:10.1038/nmeth891
| First Paragraph | Full Text (HTML / PDF) |
review article
Reading protein modifications with interaction domains
Bruce T. Seet, Ivan Dikic, Ming-Ming Zhou and Tony Pawson
Nature Reviews Molecular Cell Biology 7, 473–483
(July 2006); doi:10.1038/nrm1960
| Summary | Full Text (HTML / PDF) |
article
Monitoring dynamic protein interactions with photoquenching FRET
Ignacio A Demarco, Ammasi Periasamy, Cynthia F Booker & Richard N Day
Nature Methods 3, 519–524
(July 2006); doi:10.1038/nmeth889
| Summary | Full Text (HTML / PDF) |
June
technical report
Live imaging of lymphatic development in the zebrafish
Karina Yaniv, Sumio Isogai, Daniel Castranova, Louis Dye, Jiro Hitomi & Brant M Weinstein
Nature Medicine 12, 711 - 716
(June 2006); doi:10.1038/nm1427
| Summary | Full Text (HTML / PDF) |
letter
Clonal analysis reveals a common progenitor for thymic cortical and medullary epithelium
Simona W. Rossi, William E. Jenkinson, Graham Anderson and Eric J. Jenkinson
Nature 441, 988–991
(22 June 2006); doi:10.1038/nature04813
| First Paragraph | Full Text (HTML / PDF) |
letter
Golgi maturation visualized in living yeast
Eugene Losev, Catherine A. Reinke, Jennifer Jellen, Daniel E. Strongin, Brooke J. Bevis and Benjamin S. Glick
Nature 441, 1002–1006
(22 June 2006); doi:10.1038/nature04717
| First Paragraph | Full Text (HTML / PDF) |
letter
Live imaging of yeast Golgi cisternal maturation
Kumi Matsuura-Tokita, Masaki Takeuchi, Akira Ichihara, Kenta Mikuriya and Akihiko Nakano
Nature 441, 1007–1010
(22 June 2006); doi:10.1038/nature04737
| First Paragraph | Full Text (HTML / PDF) |
letter
A microengraving method for rapid selection of single cells producing antigen-specific antibodies
J Christopher Love, Jehnna L Ronan, Gijsbert M Grotenbreg, Annemarthe G van der Veen & Hidde L Ploegh
Nature Biotechnology 24, 703 - 707
(June 2006); doi:10.1038/nbt1210
| Summary | Full Text (HTML / PDF) |
letter
Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR
Cynthia H Collins, Jared R Leadbetter & Frances H Arnold
Nature Biotechnology 24, 708–712
(June 2006); doi:10.1038/nbt1209
| First Paragraph | Full Text (HTML / PDF) |
news and views
A luxRury of synthetic signals
Stephen W Michnick
Nature Biotechnology 24, 658–660
(June 2006); doi:10.1038/nbt0606-658
| First Paragraph | Full Text (HTML / PDF) |
article
Noise in protein expression scales with natural protein abundance
Arren Bar-Even, Johan Paulsson, Narendra Maheshri, Miri Carmi, Erin O'Shea, Yitzhak Pilpel and Naama Barkai
Nature Genetics 38, 636–643
(June 2006); doi:10.1038/ng1807
| First Paragraph | Full Text (HTML / PDF) |
news and views
Predictable trends in protein noise
Craig R Roy and Dario S Zamboni
Nature Genetics 38, 610–611
(June 2006); doi:10.1038/ng0606-610
| First Paragraph | Full Text (HTML / PDF) |
letter
Computational redesign of endonuclease DNA binding and cleavage specificity
Justin Ashworth, James J. Havranek, Carlos M. Duarte, Django Sussman, Raymond J. Monnat Jr, Barry L. Stoddard and David Baker
Nature 441, 656–659
(01 June 2006); doi:10.1038/nature04818
| First Paragraph | Full Text (HTML / PDF) |
review article
Interpreting the protein language using proteomics
Ole N. Jensen
Nature Reviews Molecular Cell Biology 7, 391–403
(June 2006); doi:10.1038/nrm1939
| Summary | Full Text (HTML / PDF) |
May
letter
Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways
Dirk Grimm, Konrad L. Streetz, Catherine L. Jopling, Theresa A. Storm, Kusum Pandey, Corrine R. Davis, Patricia Marion, Felix Salazar and Mark A. Kay
Nature 441, 537–541
(25 May 2006); doi:10.1038/nature04791
| First Paragraph | Full Text (HTML / PDF) |
article
Metabolic biotinylation of cell surface receptors for in vivo imaging
Bakhos A Tannous, Jan Grimm, Katherine F Perry, John W Chen, Ralph Weissleder & Xandra O Breakefield
Nature Methods 3, 391–396
(May 2006); doi:10.1038/nmeth875
| Summary | Full Text (HTML / PDF) |
review
High–throughput RNAi screening in cultured cells: a user's guide
Christophe J. Echeverri and Norbert Perrimon
Nature Reviews Genetics 7, 373–384
(May 2006); doi:10.1038/nrg1836
| Summary | Full Text (HTML / PDF) |
letter
A fluorescent variant of a protein from the stony coral Montipora facilitates dual–color single–laser fluorescence cross–correlation spectroscopy
Takako Kogure, Satoshi Karasawa, Toshio Araki, Kenta Saito, Masataka Kinjo & Atsushi Miyawaki
Nature Biotechnology 24, 577–581
(May 2006); doi:10.1038/nbt1207
| Summary | Full Text (HTML / PDF) |
article
Fluorescent cell barcoding in flow cytometry allows high–throughput drug screening and signaling profiling
Peter O Krutzik, Garry P Nolan
Nature Methods 3, 361–368
(May 2006); doi:10.1038/nmeth872
| Summary | Full Text (HTML / PDF) |
letter
Stable expression of shRNAs in human CD34+ progenitor cells can avoid induction of interferon responses to siRNAs in vitro
Marjorie A Robbins, Mingjie Li, Irene Leung, Haitang Li, Doris V Boyer, Yong Song, Mark A Behlke & John J Rossi
Nature Biotechnology 24, 566–571
(May 2006); doi:10.1038/nbt1206
| Summary | Full Text (HTML / PDF) |
news and views
RNA interference below the immune radar
Mouldy Sioud
Nature Biotechnology 24, 521–522
(May 2006); doi:10.1038/nbt0506–521
| Summary | Full Text (HTML / PDF) |
article
High-throughput RNAi screening by time-lapse imaging of live human cells
Beate Neumann, Michael Held, Urban Liebel, Holger Erfle, Phill Rogers, Rainer Pepperkok & Jan Ellenberg
Nature Methods 3, 385–390
(May 2006) | doi:10.1038/nmeth876
| First Paragraph | Full Text (HTML / PDF) |
April
review article
Multiplexed protein measurement: technologies and applications of protein and antibody arrays
Stephen F. Kingsmore
Nature Reviews Drug Discovery 5, 310–321
(April 2006); doi:10.1038/nrd2006
Nature Reviews Drug Discovery published online
| Summary | Full Text (HTML / PDF) |
article
Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
Vsevolod V Belousov, Arkady F Fradkov, Konstantin A Lukyanov, Dmitry B Staroverov, Konstantin S Shakhbazov, Alexey V Terskikh & Sergey Lukyanov
Nature Methods 3, 281–286
(April 2006); doi:10.1038/nmeth866
| Summary | Full Text (HTML / PDF) |
article
Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
Thomas S Wehrman, Georges von Degenfeld, Peter O Krutzik, Garry P Nolan & Helen M Blau
Nature Methods 3, 295–301
(April 2006); doi:10.1038/nmeth868
| Summary | Full Text (HTML / PDF) |
letter
Spatiotemporal dynamics of RhoA activity in migrating cells
Olivier Pertz , Louis Hodgson , Richard L. Klemke and Klaus M. Hahn
Nature 440, 1069–1072
(20 April 2006); doi:10.1038/nature04665
| First Paragraph | Full Text (HTML / PDF) |
letter
Functional classification of drugs by properties of their pairwise interactions
Pamela Yeh, Ariane I Tschumi & Roy Kishony
Nature Genetics 38, 489–494
(April 2006); doi:10.1038/ng1755
| Summary | Full Text (HTML / PDF) |
article
Slowed diffusion in tumors revealed by microfiberoptic epifluorescence photobleaching
Jay R Thiagarajah, Jung Kyung Kim, Mazin Magzoub & A S Verkman
Nature Methods 3, 275–280
(April 2006); doi:10.1038/nmeth863
| Summary | Full Text (HTML / PDF) |
letter
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
Katrin I. Willig, Silvio O. Rizzoli, Volker Westphal, Reinhard Jahn and Stefan W. Hell
Nature 440, 935–939
(13 April 2006); doi:10.1038/nature04592
| First Paragraph | Full Text (HTML / PDF) |
news and views
Biological imaging: The diffraction barrier broken
Garth J. Simpson
Nature 440, 879–880
(13 April 2006); doi:10.1038/440879a
| Full Text (HTML / PDF) |
letter
Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR
Adam Lange, Karin Giller, Sönke Hornig, Marie-France Martin-Eauclaire, Olaf Pongs, Stefan Becker and Marc Baldus
Nature 440, 959–962
(13 April 2006); doi:10.1038/nature04649
| First Paragraph | Full Text (HTML / PDF) |
review article
Local force and geometry sensing regulate cell functions
Viola Vogel and Michael Sheetz
Nature Reviews Molecular Cell Biology 7, 265–275
(April 2006); doi:10.1038/nrm1890
| Summary | Full Text (HTML / PDF) |
March
letter
Analysis of a RanGTP-regulated gradient in mitotic somatic cells
Petr Kaláb, Arnd Pralle, Ehud Y. Isacoff, Rebecca Heald and Karsten Weis
Nature 440, 697–701
(30 March 2006); doi:10.1038/nature04589
| First Paragraph | Full Text (HTML / PDF) |
review article
A visual approach to proteomics
Stephan Nickell, Christine Kofler, Andrew P. Leis and Wolfgang Baumeister
Nature Reviews Molecular Cell Biology 7, 225–230
(30 March 2006); doi:10.1038/nrm1861
| First Paragraph | Full Text (HTML / PDF) |
letter
Human chromosome 11 DNA sequence and analysis including novel gene identification
Todd D. Taylor, Hideki Noguchi, Yasushi Totoki, Atsushi Toyoda, Yoko Kuroki, Ken Dewar, Christine Lloyd, Takehiko Itoh, Tadayuki Takeda, Dae-Won Kim, Xinwei She, Karen F. Barlow, Toby Bloom, Elspeth Bruford, Jean L. Chang, Christina A. Cuomo, Evan Eichler, Michael G. FitzGerald, David B. Jaffe, Kurt LaButti, Robert Nicol, Hong-Seog Park, Christopher Seaman, Carrie Sougnez, Xiaoping Yang, Andrew R. Zimmer, Michael C. Zody, Bruce W. Birren, Chad Nusbaum, Asao Fujiyama, Masahira Hattori, Jane Rogers, Eric S. Lander and Yoshiyuki Sakaki
Nature 440, 497–500
(23 March 2006); doi:10.1038/nature04632
| Summary | Full Text (HTML / PDF) |
review article
Cell-signalling dynamics in time and space
Boris N. Kholodenko
Nature Reviews Molecular Cell Biology 7, 165–176
(March 2006); doi:10.1038/nrm1838
| Summary | Full Text (HTML / PDF) |
article
Viral nanoparticles as tools for intravital vascular imaging
John D Lewis, Giuseppe Destito, Andries Zijlstra, Maria J Gonzalez, James P Quigley, Marianne Manchester and Heidi Stuhlmann
Nature Medicine 12, 354–360
(March 2006); doi:10.1038/nm1368
| Abstract | Full Text (HTML/PDF) |
news and views
The selfish stem cell
Peter W Andrews
Nature Biotechnology 24, 325–326
(March 2006); doi:10.1038/nbt0306-325
| | Full Text (HTML/PDF) |
letter
Stochastic protein expression in individual cells at the single molecule level
Long Cai, Nir Friedman and X. Sunney Xie
Nature 440, 358–362
(16 March 2006); doi:10.1038/nature04599
| First Paragraph | Full Text (HTML / PDF) |
review article
Building mammalian signalling pathways with RNAi screens
Jason Moffat and David M. Sabatini
Nature Reviews Molecular Cell Biology 7, 177–187
(March 2006); doi:10.1038/nrm1860
| Summary | Full Text (HTML / PDF) |
review article
Microarray technology: beyond transcript profiling and genotype analysis
Jörg D. Hoheisel
Nature Reviews Genetics 7, 200–210
(March 2006); doi:10.1038/nrg1809
| Summary | Full Text (HTML / PDF) |
review article
Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET)
Kevin D G Pfleger and Karin A Eidne
Nature Methods 3, 165–174
(March 2006); doi:10.1038/nmeth841
| Abstract | Full Text (HTML/PDF) |
news and views
Pooling in systems biology becomes smart
Nicolas Thierry-Mieg
Nature Methods 3, 161–162
(March 2006); doi:10.1038/nmeth0306-161
| Full Text (HTML/PDF) |
article
A pooling-deconvolution strategy for biological network elucidation
Fulai Jin, Tony Hazbun, Gregory A Michaud, Michael Salcius, Paul F Predki, Stanley Fields and Jing Huang
Nature Methods 3, 183–189
(March 2006); doi:10.1038/nmeth859
| Abstract | Full Text (HTML/PDF) |
article
Optimal isotope labelling for NMR protein structure determinations
Masatsune Kainosho, Takuya Torizawa, Yuki Iwashita, Tsutomu Terauchi, Akira Mei Ono and Peter Güntert
Nature 440, 52–57
(02 March 2006); doi:10.1038/nature04525
| Summary | Full Text (HTML / PDF) |
news and views
Structural biology: Designer labels
Stanley J. Opella
Nature 440, 40
(02 March 2006); doi:10.1038/440040a
| Full Text (HTML / PDF) |
article
T cells use two directionally distinct pathways for cytokine secretion
Morgan Huse, Björn F Lillemeier, Michael S Kuhns, Daniel S Chen and Mark M Davis
Nature Immunology 7, 247–255
(March 2006); doi:10.1038/ni1304
| Abstract | Full Text (HTML/PDF) |
article
Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization
Holger Lorenz, Dale W Hailey and Jennifer Lippincott-Schwartz
Nature Methods 3, 205–210
(March 2006); doi:10.1038/nmeth857
| Full Text (HTML/PDF) |
February
letter
Purification and unique properties of mammary epithelial stem cells
John Stingl, Peter Eirew, Ian Ricketson, Mark Shackleton, François Vaillant, David Choi, Haiyan I. Li and Connie J. Eaves
Nature 439, 993–997
(23 February 2006); doi:10.1038/nature04496
| First Paragraph | Full Text (HTML / PDF) |
letter
Identification of pathways regulating cell size and cell-cycle progression by RNAi
Mikael Björklund , Minna Taipale, Markku Varjosalo, Juha Saharinen, Juhani Lahdenperä and Jussi Taipale
Nature 439, 1009–1013
(23 February 2006); doi:10.1038/nature04469
| First Paragraph | Full Text (HTML / PDF) |
review article
Mapping normal and cancer cell signalling networks: towards single-cell proteomics
Jonathan M. Irish, Nikesh Kotecha and Garry P. Nolan
Flow cytometry can be used to map signalling networks in individual cancer cells. This form of 'single-cell proteomics' can reveal important new information about the pathways that are activated in therapy-resistant cells and provide biomarkers for use in diagnosis and determining prognosis.
Nature Reviews Cancer 6, 146–155
(February 2006); doi:10.1038/nrc1804
| Summary | Full Text (HTML / PDF) |
article
Proteome survey reveals modularity of the yeast cell machinery
Anne-Claude Gavin, Patrick Aloy, Paola Grandi, Roland Krause, Markus Boesche, Martina Marzioch, Christina Rau1, Lars Juhl Jensen, Sonja Bastuck, Birgit Dümpelfeld, Angela Edelmann, Marie-Anne Heurtier, Verena Hoffman, Christian Hoefert, Karin Klein, Manuela Hudak, Anne-Marie Michon, Malgorzata Schelder, Markus Schirle, Marita Remor, Tatjana Rudi, Sean Hooper, Andreas Bauer, Tewis Bouwmeester, Georg Casari, Gerard Drewes, Gitte Neubauer, Jens M. Rick, Bernhard Kuster, Peer Bork, Robert B. Russell and Giulio Superti-Furga
Nature Advance online publication
(22 January 2006); doi:10.1038/nature04561
| First Paragraph | Full Text (HTML / PDF) |
letter
Quantitative imaging of Plasmodium transmission from mosquito to mammal
Rogerio Amino, Sabine Thiberge, Béatrice Martin, Susanna Celli, Spencer Shorte, Friedrich Frischknecht and Robert Ménard
Nature Medicine 12, 220–224
(February 2006); doi:10.1038/nm1350
| First Paragraph | Full Text (HTML/PDF) |
news and views
Malaria parasites up close
Malcolm K Jones and Michael F Good
Nature Medicine 12, 170–171
(February 2006); doi:10.1038/nm0206-170
| Full Text (HTML/PDF) |
brief communication
Derivation of human embryonic stem cells in defined conditions
Tenneille E Ludwig, Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas and James A Thomson
Nature Biotechnology 24, 185–187
(February 2006); doi:10.1038/nbt1177
| First Paragraph | Full Text (HTML/PDF) |
letter
Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
Yuriy Kirichok, Betsy Navarro and David E. Clapham
Nature 439, 737–740
(09 February 2006); doi:10.1038/nature04417
| First Paragraph | Full Text (HTML / PDF) |
protocol
Protein-protein interaction assays: eliminating false positive interactions
Tuan N Nguyen and James A Goodrich
Nature Methods 3, 135–139
(February 2006); doi:10.1038/nmeth0206-135
| Full Text (HTML/PDF) |
article
A versatile tool for conditional gene expression and knockdown
Jolanta Szulc, Maciej Wiznerowicz, Marc-Olivier Sauvain, Didier Trono and Patrick Aebischer
Nature Methods 3, 109–116
(February 2006); doi:10.1038/nmeth846
| Abstract | Full Text (HTML/PDF) |
January
brief communication
Production of a recombinant bacterial lipoprotein in higher plant chloroplasts
Karin Glenz, Bernadette Bouchon, Thomas Stehle, Reinhard Wallich, Markus M Simon and Heribert Warzecha
Nature Biotechnology 24, 76–77
(January 2006); doi:10.1038/nbt1170
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letter
Oscillations of cyclic AMP in hormone-stimulated insulin-secreting β-cells
Oleg Dyachok, Yegor Isakov, Jenny Sågetorp and Anders Tengholm
Nature 439, 349–352
(19 January 2006); doi:10.1038/nature04410
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letter
In situ trapping of activated initiator caspases reveals a role for caspase-2 in heat shock-induced apoptosis
Shine Tu, Gavin P. McStay, Louis-Martin Boucher, Tak Mak, Helen M. Beere and Douglas R. Green
Nature Cell Biology 8, 72–77
(January 2006); doi:10.1038/ncb1340
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letter
Microarray data analysis: from disarray to consolidation and consensus
David B. Allison , Xiangqin Cui, Grier P. Page and Mahyar Sabripour
Nature Reviews Genetics 7, 55–65
(January 2006); doi:10.1038/nrg1749
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protocol
Strategies for protein coexpression in Escherichia coli
Niraj H Tolia and Leemor Joshua-Tor
Nature Methods 3, 55–64
(January 2006); doi:10.1038/nmeth0106-55
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classic protocol
Transfection of mammalian cells by electroporation
Nature Methods 3, 67–68
(January 2006); doi:10.1038/nmeth0106-67
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research article
A genetic strategy to treat sickle cell anemia by coregulating globin transgene expression and RNA interference
Selda Samakoglu, Leszek Lisowski, Tulin Budak-Alpdogan, Yelena Usachenko, Santina Acuto, Rosalba Di Marzo, Aurelio Maggio, Ping Zhu, John F. Tisdale, Isabelle Rivière and Michel Sadelain
Nature Biotechnology 24, 89–94
(January 2006); doi:10.1038/nbt1176
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letter
Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts
Alexander Meissner and Rudolf Jaenisch
Nature 439, 212–215
(12 January 2006); doi:10.1038/nature04257
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news and views
Medicine: Politic stem cells
Irving L. Weissman
Nature 439, 145–147
(12 January 2006); doi:10.1038/439145a
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technical report
Lipid microarrays identify key mediators of autoimmune brain inflammation
Jennifer L Kanter, Sirisha Narayana, Peggy P Ho, Ingrid Catz, Kenneth G Warren, Raymond A Sobel, Lawrence Steinman and William H Robinson
Nature Medicine 12, 138–143
(January 2006); doi:10.1038/nm1344
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article
A single-molecule method for the quantitation of microRNA gene expression
Lori A Neely, Sonal Patel, Joanne Garver, Michael Gallo, Maria Hackett, Stephen McLaughlin, Mark Nadel, John Harris, Steve Gullans and Jenny Rooke
Nature Methods 3, 41–46
(January 2006); doi:10.1038/nmeth825
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brief communication
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
Wigard P Kloosterman, Erno Wienholds, Ewart de Bruijn, Sakari Kauppinen and Ronald H A Plasterk
Nature Methods 3, 27–29
(January 2006); doi:10.1038/nmeth843
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news and views
microRNA detection comes of age
Scott M Hammond
Nature Methods 3, 12–13
(January 2006); doi:10.1038/nmeth0106-12
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letter
Generation of a functional mammary gland from a single stem cell
Mark Shackleton, François Vaillant, Kaylene J. Simpson, John Stingl, Gordon K. Smyth, Marie-Liesse Asselin-Labat, Li Wu, Geoffrey J. Lindeman and Jane E. Visvader
Nature 439, 84–88
(05 January 2006); doi:10.1038/nature04372
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article
The nitrodibenzofuran chromophore: a new caging group for ultra-efficient photolysis in living cells
Atsuya Momotake, Nicolas Lindegger, Ernst Niggli, Robert J Barsotti and Graham C R Ellis-Davies
Nature Methods 3, 35–40
(January 2006); doi:10.1038/nmeth821
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news and views
Crafting new cages
Wen-Hong Li
Nature Methods 3, 13–15
(January 2006); doi:10.1038/nmeth0106-13
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